Brief description
Glass slides were deployed at 12m and 18m3 at sites in McMurdo Sound (adjacent to McMurdo Station, Scott Base and Cape Armitage) for a period of 12 months to collect biofilm.\n\n18S rDNA sequence information was obtained for representatives of each DGGE (denaturing gradient gel electrophoresis) band at each site and depth. This data was interrogated to phyla level: cnidaria, bryozoa, protozoa, dinoflagellates, arthropods, platyhelminths and annelids were present.\n\nDensity of diatoms observed by fluorescence microscopy for each site and depth were recorded (per 100 µm2). At least 6 different diatom morphotypes were identified based on size and morphology.\n\nThe slides were also examined by performing fluorescence in situ hybridization (FISH) reactions with bacteria-specific probes (Eub338), an archaea-specific probe (ARCH915) and 9 different bacterial group-specific probes. \n\nBacterial groups identified from the sequences were: Gammaproteobacteria, SRB, Planctomycetales, Alphaproteobacteria, Archaea, Betaproteobacteria, Actinobacteria and Firmicutes, Cytophaga /Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia.\n\nThis study was the first to examine eukaryotic recruitment, potentially overlooked in visual surveys, using molecular techniques.Full description
Point of truth URL of this metadata record -http://gcmd.nasa.gov/Resources/valids/archives/keyword_list.html -
text: true
Subjects
16S rDNA |
18S rDNA |
Aquatic Ecosystems |
Arthropods |
Bacteria |
Benthic Habitat |
Biological Classification |
Biosphere |
Cnidarians |
Diatoms |
Ecological Dynamics |
Plants |
Protists |
Sponges |
sequencing |
User Contributed Tags
Login to tag this record with meaningful keywords to make it easier to discover
Identifiers
- Local : marine-eukaryotes-and-bacteria-antarctica
- URI : data.gov.au/dataset/9851d844-672b-407b-bce1-7373c6c3ed8b
