Through the National Collaborative Research Infrastructure Strategy (NCRIS), the Commonwealth Government and State Government partners have enhanced Australia's capacity to produce biofuels derived from non-food biomass in pre-commercial quantities. This project has involved the development of five integrated sites located around Australia that aim to provide Australian based researchers with subsidised access to quality facilities, technologically advanced equipment, and technical expertise required for process development and pilot scale manufacture of biofuels. One of the key objectives underpinning this project is to foster collaboration among the biofuels community by reducing duplication of research infrastructure. The project focus is on providing infrastructure for the conversion of lignocellulosic and microalgae biomass to biofuels (ethanol and biodiesel). To facilitate process development activities, laboratory infrastructure at three participating universities has been enhanced.