Data

Lucinda Jetty Coastal Observatory, HPLC Pigment and Ocean Colour Data

Integrated Marine Observing System
Brewer, Elizabeth (Point of contact, Processor of) CSIRO O&A, Information & Data Centre (Point of contact) Clementson, Lesley (Originator of)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/cd1cbb01-51af-4482-8bf6-9076f52300df&rft.title=Lucinda Jetty Coastal Observatory, HPLC Pigment and Ocean Colour Data&rft.identifier=cd1cbb01-51af-4482-8bf6-9076f52300df&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data collected from the near-water surface sampling site off the Lucinda Jetty, as part of the Lucinda Jetty Coastal Observatory. Sample collection began the 9th of January, 2014. The data can be used for Ocean Colour sensor validation. Parameters measured include the absorption coefficients of the particulate and dissolved components of the water column and the phytoplankton pigment concentration and composition.Maintenance and Update Frequency: asNeededStatement: Water samples were taken at the Lucinda Jetty Coastal Observatory and stored under cool and dark conditions until filtering took place. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory at Oceans and Atmosphere, Hobart. Pigment analysis; approximately 2 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). CDOM absorption; sample water was filtered through a 0.22 μm filter (Millipore Durapore), and the filtrate was stored in clean dry glass bottles and kept dark and cool until analysis. After equilibrating to room temperature, the CDOM absorbance of each filtrate was measured in a 10 cm pathlength quartz cell using a Cintra 404 UV/VIS spectrophotometer, with fresh Milli-QTM water (Millipore) as a reference. Spectral absorption; approximately 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a Cinta 404 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.&rft.creator=Anonymous&rft.date=2015&rft.coverage=westlimit=153.1807; southlimit=-27.3303; eastlimit=153.1807; northlimit=-27.3303&rft.coverage=westlimit=153.1807; southlimit=-27.3303; eastlimit=153.1807; northlimit=-27.3303&rft.coverage=uplimit=-1; downlimit=-1&rft.coverage=uplimit=-1; downlimit=-1&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Optics | Absorption&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Oceans | Ocean Optics | Gelbstoff&rft_subject=Earth Science | Oceans | Ocean Chemistry | Suspended Solids&rft_subject=Fluorometers&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Countries | Australia&rft_subject=Coastal Waters (Australia) | Queensland Coast, QLD&rft_subject=Lucinda Jetty Coastal Observatory&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=research vessel&rft_subject=CTD&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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This record contains data collected from the near-water surface sampling site off the Lucinda Jetty, as part of the Lucinda Jetty Coastal Observatory. Sample collection began the 9th of January, 2014. The data can be used for Ocean Colour sensor validation. Parameters measured include the absorption coefficients of the particulate and dissolved components of the water column and the phytoplankton pigment concentration and composition.

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Maintenance and Update Frequency: asNeeded
Statement: Water samples were taken at the Lucinda Jetty Coastal Observatory and stored under cool and dark conditions until filtering took place. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory at Oceans and Atmosphere, Hobart. Pigment analysis; approximately 2 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). CDOM absorption; sample water was filtered through a 0.22 μm filter (Millipore Durapore), and the filtrate was stored in clean dry glass bottles and kept dark and cool until analysis. After equilibrating to room temperature, the CDOM absorbance of each filtrate was measured in a 10 cm pathlength quartz cell using a Cintra 404 UV/VIS spectrophotometer, with fresh Milli-QTM water (Millipore) as a reference. Spectral absorption; approximately 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a Cinta 404 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.

Notes

Credit
Thomas Schroeder (CSIRO O&A Dutton Park),
Credit
Lesley Clementson (CSIRO O&A Hobart)
Credit
Vittorio Brando (CSIRO O&A Black Mountain)
Credit
Heidi Franklin (CSIRO O&A Dutton Park)

Data time period: 2014-01-09 to 2020-12-09

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153.1807,-27.3303

153.1807,-27.3303

text: westlimit=153.1807; southlimit=-27.3303; eastlimit=153.1807; northlimit=-27.3303

text: uplimit=-1; downlimit=-1

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Coastal Waters (Australia) | Queensland Coast, QLD | Countries | Australia | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Chemistry | Suspended Solids | Earth Science | Oceans | Ocean Optics | Absorption | Earth Science | Oceans | Ocean Optics | Gelbstoff | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Fluorometers | HPLC (High Performance Liquid Chromatography) | Lucinda Jetty Coastal Observatory | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Spectrophotometers | Temperature of the water body | oceans | research vessel |

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Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Data Link)

uri : https://aesop.csiro.au/

IMOS Lucinda Jetty Coastal Observatory (LJCO ) Page (Documentation Link)

uri : https://imos.org.au/facilities/srs/oceancolour

Overview coastal - ocean colour cal - val Australia (Documentation Link)

uri : https://earth.esa.int/documents/700255/1793811/Dekker_CSIRO_MVT-Oct11.pdf/02891024-6972-48a4-b13f-e22069f7d471

This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

uri : https://creativecommons.org/licenses/by/4.0/

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  • global : cd1cbb01-51af-4482-8bf6-9076f52300df
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