Data

Kautilya_PhD_Chapter 3

Commonwealth Scientific and Industrial Research Organisation
Kaksonen, Anna ; Srivastava, Kautilya ; Mathes, Falko ; Campbell, Bronwyn ; Middleton, Jen ; Pejcic, Bobby ; Tobar, Daniel ; Hovey, Renae
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25919/agnx-4578&rft.title=Kautilya_PhD_Chapter 3&rft.identifier=https://doi.org/10.25919/agnx-4578&rft.publisher=Commonwealth Scientific and Industrial Research Organisation&rft.description=Chapter 3 presents a manipulative field experiment at Herdsman Lake, an urban wetland in metropolitan Perth, examining the microbial communities that colonise plastic buried in sediment and whether they carry the genetic capacity to degrade it. Low-density polyethylene (LDPE) films were deployed as the test substrate alongside polytetrafluoroethylene (PTFE) as an inert negative control, buried for 90 days and retrieved across a time series together with paired bulk sediment samples. Genomic DNA was extracted from plastic-associated biofilms and sediment and sequenced by shotgun metagenomics. The microbes and genes associated with LDPE degradation were looked at, along with physicochemical changes of plastics&rft.creator=Kaksonen, Anna &rft.creator=Srivastava, Kautilya &rft.creator=Mathes, Falko &rft.creator=Campbell, Bronwyn &rft.creator=Middleton, Jen &rft.creator=Pejcic, Bobby &rft.creator=Tobar, Daniel &rft.creator=Hovey, Renae &rft.date=2026&rft.edition=v1&rft.coverage=115.81009999999999,-31.9269&rft_rights=Creative Commons Attribution-Noncommercial 4.0 Licence https://creativecommons.org/licenses/by-nc/4.0/&rft_rights=Data is accessible online and may be reused in accordance with licence conditions&rft_rights=All Rights (including copyright) CSIRO 2026.&rft_subject=Plastic Degradation&rft_subject=Wetland&rft_subject=LDPE&rft_subject=PTFE, MAGs&rft_subject=Microbial ecology&rft_subject=Microbiology&rft_subject=BIOLOGICAL SCIENCES&rft.type=dataset&rft.language=English Access the data
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Data is accessible online and may be reused in accordance with licence conditions

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Chapter 3 presents a manipulative field experiment at Herdsman Lake, an urban wetland in metropolitan Perth, examining the microbial communities that colonise plastic buried in sediment and whether they carry the genetic capacity to degrade it. Low-density polyethylene (LDPE) films were deployed as the test substrate alongside polytetrafluoroethylene (PTFE) as an inert negative control, buried for 90 days and retrieved across a time series together with paired bulk sediment samples. Genomic DNA was extracted from plastic-associated biofilms and sediment and sequenced by shotgun metagenomics. The microbes and genes associated with LDPE degradation were looked at, along with physicochemical changes of plastics

Available: 2026-09-04

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115.8101,-31.9269

115.8101,-31.9269

Subjects
Biological Sciences | LDPE | Microbial Ecology | Microbiology | PTFE, MAGs | Plastic Degradation | Wetland |

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Identifiers
ACN 633 798 857