Data

Interstitial Biofilm Formation in P. Aeruginosa (data for Supplementary 3, Figure 3G in related publication): time series images

University of Technology Sydney
Associate Professor Cynthia Whitchurch (Associated with) Cynthia Whitchurch (Associated with) Doctor Lynne Turnbull (Aggregated by) Lynne Turnbull (Aggregated by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://hdl.handle.net/11057/ds51a904c6b486798b9437b5f5afb982b943440f32&rft.title=Interstitial Biofilm Formation in P. Aeruginosa (data for Supplementary 3, Figure 3G in related publication): time series images&rft.identifier=http://hdl.handle.net/11057/ds51a904c6b486798b9437b5f5afb982b943440f32&rft.publisher=University of Technology Sydney&rft.description=This folder contains 1000 tiff images. These tif images comprise a time series captured at a rate of 1 frame every 2 seconds. These images were captured using an inverted microscope with a 100x phase oil-immersion objective (Olympus IX-71) fitted with an FViewII (Soft Imaging Systems) monochromatic camera. Selected frames from this movie were used in the creation of Figure 3G in the related publication, showing phase-contrast images of the assembly of a small bulldozer aggregate breaking away from an established trail in an interstitial biofilm to form a new trail that intersects with another newly formed trail. (Scale bar, 5 μm.) Interstitial biofilms of the bacteria Pseudomonas aeruginosa were cultured on 0.4xLB-Lennox solidified with 8 g/L gellan gum (twitching motility gellan gum, TMGG). Molten TMGG was poured over sterile slides and solidified at room temperature. Slides were inoculated, covered with a sterile coverslip, and incubated in humid conditions at 37 °C. Media was supplemented with 100 Kunitz units per mL DNaseI (D5025, Sigma Aldrich) or the enzyme storage buffer [50% vol/vol glycerol, 10 mM MgCl2, 10 mM CaCl2, 10 mM Tris•HCl]. &rft.creator=Doctor Lynne Turnbull&rft.creator=Lynne Turnbull&rft.date=2014&rft.relation=http://www.pnas.org/content/early/2013/06/21/1218898110&rft_rights=Copyright University of Technology, Sydney, 2014&rft_rights=CC BY: Attribution 3.0 AU http://creativecommons.org/licenses/by/3.0/au&rft_subject=bacterial biofilms&rft_subject=interstitial biofilm formation&rft_subject=microbial imaging&rft_subject=extracellular DNA&rft_subject=Pseudomonas Aeruginosa&rft_subject=Medical Bacteriology&rft_subject=MEDICAL AND HEALTH SCIENCES&rft_subject=MEDICAL MICROBIOLOGY&rft_subject=Bacteriology&rft_subject=BIOLOGICAL SCIENCES&rft_subject=MICROBIOLOGY&rft_subject=Infectious Diseases&rft_subject=HEALTH&rft_subject=CLINICAL HEALTH (ORGANS, DISEASES AND ABNORMAL CONDITIONS)&rft_subject=Strategic basic research&rft.type=dataset&rft.language=English Access the data

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Microbial Imaging Facility,Level 6, Building 4 (CB04) UTS City Campus Cnr Harris and Thomas Streets Ultimo, NSW 2007 Australia

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Copyright University of Technology, Sydney, 2014

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This folder contains 1000 tiff images. These tif images comprise a time series captured at a rate of 1 frame every 2 seconds. These images were captured using an inverted microscope with a 100x phase oil-immersion objective (Olympus IX-71) fitted with an FViewII (Soft Imaging Systems) monochromatic camera.
Selected frames from this movie were used in the creation of Figure 3G in the related publication, showing phase-contrast images of the assembly of a small bulldozer aggregate breaking away from an established trail in an interstitial biofilm to form a new trail that intersects with another newly formed trail. (Scale bar, 5 μm.)
Interstitial biofilms of the bacteria Pseudomonas aeruginosa were cultured on 0.4xLB-Lennox solidified with 8 g/L gellan gum (twitching motility gellan gum, TMGG). Molten TMGG was poured over sterile slides and solidified at room temperature. Slides were inoculated, covered with a sterile coverslip, and incubated in humid conditions at 37 °C. Media was supplemented with 100 Kunitz units per mL DNaseI (D5025, Sigma Aldrich) or the enzyme storage buffer [50% vol/vol glycerol, 10 mM MgCl2, 10 mM CaCl2, 10 mM Tris•HCl].

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Related Publications

  • Associated with Gloag, E.S., Turnbull, L., Huang, A., Vallotton, P., Wang, H., Nolan, L.M., Mililli, L., Hunt, C., Lu, J., Osvath, S.R., Monahan, L.G., Cavaliere, R., Charles, I.G., Wand, M.P., Gee, M.L., Ranganathan, P. & Whitchurch, C.B. (2013) Self-organization of bacterial biofilms is facilitated by extracellular DNA, Proceedings of the National Academy of the Sciences USA, 110 (28): 11541-11546.
    uri : www.pnas.org/content/early/2013/06/21/1218898110

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Biological Sciences | Bacteriology | Clinical Health (Organs, Diseases and Abnormal Conditions) | Health | Infectious Diseases | Medical and Health Sciences | Medical Microbiology | Microbiology | Medical Bacteriology | Pseudomonas Aeruginosa | Strategic basic research | bacterial biofilms | extracellular DNA | interstitial biofilm formation | microbial imaging |

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