Full description
This folder contains 1000 tiff images. These tif images comprise a time series captured at a rate of 1 frame every 2 seconds. These images were captured using an inverted microscope with a 100x phase oil-immersion objective (Olympus IX-71) fitted with an FViewII (Soft Imaging Systems) monochromatic camera.This movie is associated with the lower right panel of Figure S5 in the related publication. Analysis was done using the protocol described in the related publication (Supplementary 1).
Interstitial biofilms of the bacteria Pseudomonas aeruginosa were cultured on 0.4xLB-Lennox solidified with 8 g/L gellan gum (twitching motility gellan gum, TMGG). Molten TMGG was poured over sterile slides and solidified at room temperature. Slides were inoculated, covered with a sterile coverslip, and incubated in humid conditions at 37 °C. Media was supplemented with 100 Kunitz units per mL DNaseI (D5025, Sigma Aldrich) or the enzyme storage buffer [50% vol/vol glycerol, 10 mM MgCl2, 10 mM CaCl2, 10 mM Tris•HCl].
Subjects
Biological Sciences |
Bacteriology |
Clinical Health (Organs, Diseases and Abnormal Conditions) |
Health |
Infectious Diseases |
Medical and Health Sciences |
Medical Microbiology |
Microbiology |
Medical Bacteriology |
Pseudomonas Aeruginosa |
Strategic basic research |
bacterial biofilms |
extracellular DNA |
interstitial biofilm formation |
microbial imaging |
User Contributed Tags
Login to tag this record with meaningful keywords to make it easier to discover
Identifiers