Data

Interstitial Biofilm Formation in P. Aeruginosa (data for Figure S5-lower right panel in related publication) - time series images

University of Technology Sydney
Associate Professor Cynthia Whitchurch (Associated with) Cynthia Whitchurch (Associated with) Doctor Lynne Turnbull (Aggregated by) Lynne Turnbull (Aggregated by)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://hdl.handle.net/11057/dsa3dbc2582073379215ce9a543b16e9b843d72dca&rft.title=Interstitial Biofilm Formation in P. Aeruginosa (data for Figure S5-lower right panel in related publication) - time series images&rft.identifier=http://hdl.handle.net/11057/dsa3dbc2582073379215ce9a543b16e9b843d72dca&rft.publisher=University of Technology Sydney&rft.description=This folder contains 1000 tiff images. These tif images comprise a time series captured at a rate of 1 frame every 2 seconds. These images were captured using an inverted microscope with a 100x phase oil-immersion objective (Olympus IX-71) fitted with an FViewII (Soft Imaging Systems) monochromatic camera. This movie is associated with the lower right panel of Figure S5 in the related publication. Analysis was done using the protocol described in the related publication (Supplementary 1). Interstitial biofilms of the bacteria Pseudomonas aeruginosa were cultured on 0.4xLB-Lennox solidified with 8 g/L gellan gum (twitching motility gellan gum, TMGG). Molten TMGG was poured over sterile slides and solidified at room temperature. Slides were inoculated, covered with a sterile coverslip, and incubated in humid conditions at 37 °C. Media was supplemented with 100 Kunitz units per mL DNaseI (D5025, Sigma Aldrich) or the enzyme storage buffer [50% vol/vol glycerol, 10 mM MgCl2, 10 mM CaCl2, 10 mM Tris•HCl]. &rft.creator=Doctor Lynne Turnbull&rft.creator=Lynne Turnbull&rft.date=2014&rft.relation=http://www.pnas.org/content/early/2013/06/21/1218898110&rft_rights=Copyright University of Technology, Sydney, 2014&rft_rights=CC BY: Attribution 3.0 AU http://creativecommons.org/licenses/by/3.0/au&rft_subject=interstitial biofilm formation&rft_subject=bacterial biofilms&rft_subject=extracellular DNA&rft_subject=microbial imaging&rft_subject=Pseudomonas Aeruginosa&rft_subject=Bacteriology&rft_subject=BIOLOGICAL SCIENCES&rft_subject=MICROBIOLOGY&rft_subject=Medical Bacteriology&rft_subject=MEDICAL AND HEALTH SCIENCES&rft_subject=MEDICAL MICROBIOLOGY&rft_subject=Infectious Diseases&rft_subject=HEALTH&rft_subject=CLINICAL HEALTH (ORGANS, DISEASES AND ABNORMAL CONDITIONS)&rft_subject=Strategic basic research&rft.type=dataset&rft.language=English Access the data

Please use the contact information below to request access to this data.

Contact Information

Microbial Imaging Facility,Level 6, Building 4 (CB04) UTS City Campus Cnr Harris and Thomas Streets Ultimo, NSW 2007 Australia

Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

Open Licence view details
CC-BY

CC BY: Attribution 3.0 AU
http://creativecommons.org/licenses/by/3.0/au

Copyright University of Technology, Sydney, 2014

Access:

Other view details

This data is under temporary embargo. Please contact Assoc. Professor Cynthia Whitchurch to negotiate access

Full description

This folder contains 1000 tiff images. These tif images comprise a time series captured at a rate of 1 frame every 2 seconds. These images were captured using an inverted microscope with a 100x phase oil-immersion objective (Olympus IX-71) fitted with an FViewII (Soft Imaging Systems) monochromatic camera.
This movie is associated with the lower right panel of Figure S5 in the related publication. Analysis was done using the protocol described in the related publication (Supplementary 1).
Interstitial biofilms of the bacteria Pseudomonas aeruginosa were cultured on 0.4xLB-Lennox solidified with 8 g/L gellan gum (twitching motility gellan gum, TMGG). Molten TMGG was poured over sterile slides and solidified at room temperature. Slides were inoculated, covered with a sterile coverslip, and incubated in humid conditions at 37 °C. Media was supplemented with 100 Kunitz units per mL DNaseI (D5025, Sigma Aldrich) or the enzyme storage buffer [50% vol/vol glycerol, 10 mM MgCl2, 10 mM CaCl2, 10 mM Tris•HCl].

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related Publications

  • Associated with Gloag, E.S., Turnbull, L., Huang, A., Vallotton, P., Wang, H., Nolan, L.M., Mililli, L., Hunt, C., Lu, J., Osvath, S.R., Monahan, L.G., Cavaliere, R., Charles, I.G., Wand, M.P., Gee, M.L., Ranganathan, P. & Whitchurch, C.B. (2013) Self-organization of bacterial biofilms is facilitated by extracellular DNA, Proceedings of the National Academy of the Sciences USA, 110 (28): 11541-11546.
    uri : www.pnas.org/content/early/2013/06/21/1218898110

Related Organisations

Related People

Related Services

Subjects
Biological Sciences | Bacteriology | Clinical Health (Organs, Diseases and Abnormal Conditions) | Health | Infectious Diseases | Medical and Health Sciences | Medical Microbiology | Microbiology | Medical Bacteriology | Pseudomonas Aeruginosa | Strategic basic research | bacterial biofilms | extracellular DNA | interstitial biofilm formation | microbial imaging |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Identifiers
Similar data you may be interested in: