Brief description
To investigate seasonal variability in biomarkers of exposure in fish, black bream (Acanthopagrus butcheri) were collected from seven sites in the Swan-Canning Estuary in winter 2000 and in summer 2001 to evaluate the interseasonal variability of biochemical markers of exposure in the black breamLineage
Maintenance and Update Frequency: notPlanned
Statement: - Fish and Sample Collection -
One hundred and six black bream were collected during winter 2000, and 137 were captured in summer 2001 at seven sites (see thumbnail). Water quality was measured for temperature, pH, and salinity 1.5?2.0 m below the surface of the water, using a TPS WP-81 Conductivity- Salinity-pH-Temperature Meter at each site during fish collection. Dissolved oxygen Tissue collection was undertaken using the same techniques described in Webb and Gagnon (2002) (Chapter 3 of thesis). The biopsies collected were analyzed for the following biomarkers: ECOD and EROD activity, serum sorbitol dehydrogenase (s-SDH) activity, and PAH biliary metabolites of the naphthalene-, pyrene-, and benzo(a)pyrene types. These biomarkers are
known to be responsive to the contaminants in the estuary (Webb and Gagnon, 2002). In addition, they are more sensitive than is chemical analysis for petroleum hydrocarbon detection and indicate bioavailability and uptake by fish. EROD activity has been shown to be influenced by the sex of the fish (Hodson et al., 1991; Goksoyr and Forlin, 1992;
Webb and Gagnon, 2002). As a consequence, ECOD activity was assessed as a possible alternative to EROD activity, as other studies have indicated that ECOD is not affected by sex in some species of fish (Holdway et al., 1994; Machala et al., 1997).
The weight of each liver and gonad was recorded. The Condition Factor (CF), Liver Somatic Index (LSI) and Gonadosomatic Index (GSI) were calculated according to the equations:
(1) CF = [(BW - GW)/TL power of 3] × 100
(2) LSI = (LW/CW) × 100
(3) GSI = (GW/CW) × 100
- where BW = total body weight, GW = gonad weight, TL = total length, LW = liver weight and CW = carcass weight. The condition factor is based on gonad-free weight to avoid any bias due to variations in sexual maturation, and the LSI and GSI are based on carcass weight to avoid bias due to variable levels of fat in the gonads and intestines, and variable gonad weight (Hodson et al., 1991).
For methods on determining biomarkers - the mixed function oxygenase (MFO) enzyme ethoxyresorufin-O-deethylase (EROD) activity and PAH bile metabolites, see Methods section of the journal article.
One hundred and six black bream were collected during winter 2000, and 137 were captured in summer 2001 at seven sites (see thumbnail). Water quality was measured for temperature, pH, and salinity 1.5?2.0 m below the surface of the water, using a TPS WP-81 Conductivity- Salinity-pH-Temperature Meter at each site during fish collection. Dissolved oxygen Tissue collection was undertaken using the same techniques described in Webb and Gagnon (2002) (Chapter 3 of thesis). The biopsies collected were analyzed for the following biomarkers: ECOD and EROD activity, serum sorbitol dehydrogenase (s-SDH) activity, and PAH biliary metabolites of the naphthalene-, pyrene-, and benzo(a)pyrene types. These biomarkers are
known to be responsive to the contaminants in the estuary (Webb and Gagnon, 2002). In addition, they are more sensitive than is chemical analysis for petroleum hydrocarbon detection and indicate bioavailability and uptake by fish. EROD activity has been shown to be influenced by the sex of the fish (Hodson et al., 1991; Goksoyr and Forlin, 1992;
Webb and Gagnon, 2002). As a consequence, ECOD activity was assessed as a possible alternative to EROD activity, as other studies have indicated that ECOD is not affected by sex in some species of fish (Holdway et al., 1994; Machala et al., 1997).
The weight of each liver and gonad was recorded. The Condition Factor (CF), Liver Somatic Index (LSI) and Gonadosomatic Index (GSI) were calculated according to the equations:
(1) CF = [(BW - GW)/TL power of 3] × 100
(2) LSI = (LW/CW) × 100
(3) GSI = (GW/CW) × 100
- where BW = total body weight, GW = gonad weight, TL = total length, LW = liver weight and CW = carcass weight. The condition factor is based on gonad-free weight to avoid any bias due to variations in sexual maturation, and the LSI and GSI are based on carcass weight to avoid bias due to variable levels of fat in the gonads and intestines, and variable gonad weight (Hodson et al., 1991).
For methods on determining biomarkers - the mixed function oxygenase (MFO) enzyme ethoxyresorufin-O-deethylase (EROD) activity and PAH bile metabolites, see Methods section of the journal article.
Issued: 13 05 2005
Data time period: 2000-08 to 2001-02
text: westlimit=115.75; southlimit=-32.1; eastlimit=116; northlimit=-31.85
Subjects
37 353003 |
ANIMALS/VERTEBRATES |
Acanthopagrus butcheri |
BIOLOGICAL CLASSIFICATION |
Black bream |
COASTAL PROCESSES |
Contaminants |
EARTH SCIENCE |
Estuaries |
Fish |
OCEANS |
TERRESTRIAL HYDROSPHERE |
WATER QUALITY/WATER CHEMISTRY |
aquatic toxicology |
bioindicators |
biomarkers |
oceans |
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Other Information
(PhD Thesis)
uri :
http://adt.curtin.edu.au/theses/available/adt-WCU20061204.135553/
global : d5aac340-5c41-11dc-af0d-00188b4c0af8
Identifiers
- global : d1bce600-5f5c-11dc-a47f-00188b4c0af8
