In vivo percutaneous absorption data for model chemicals in Rhinella marina: chemicals formulated in Amphibian Ringer’s Solution

Brief description

In vivo percutaneous absorption kinetics of caffeine, benzoic acid, and ibuprofen in the terrestrial toad Rhinella marina.

Full description

Previous studies by the authors measured in vitro absorption kinetics of model chemicals through Rh. marina skin when the chemicals were formulated as saturated solutions in Amphibian Ringers Solution. However, while there is evidence that in vitro absorption kinetics provide relatively good correlation with the in vivo situation in mammals, it is unknown how well in vitro data matches the in vivo situation in frogs. This dataset provides in vivo absorption kinetics for three model chemicals - benzoic acid, caffeine, and ibuprofen – formulated in Amphibian Ringer’s solution, in the cane toad Rhinella marina. 

Each chemical was formulated individually as a saturated solution (infinite dose) in Amphibian Ringer’s solution. 25 ml of the resultant solution was provided in a bath formulation to each animal. This volume ensured exposure of solution to the entire ventral pelvis in each animal.  Four animals were sacrificed at each sampling time and a cardiac blood sample taken. Following extraction of serum, chemical quantification was undertaken using UHPLC. Chemical flux was determined from the steady-state slope of the cumulative absorption vs time plot. 

Data includes a spreadsheet (comma-separated values / .csv file) providing individual animal information (including animal ID, sex, snout-vent length and weight), chemical information including name and concentration, and serum concentration of chemical when sampled.

Column headings for the spreadsheet are explained below:

Chemical: chemical applied to the skin (caffeine/benzoic acid/ibuprofen)

Conc: concentration of chemical applied (microgram/ml)

AnimalID: individual animal identifier

Weight: frog weight in grams

SVL: animal snout-vent length (millimetres)

SA.total: total skin surface area calculated using [SAtotal=9.9*(animal weight^0.56)] (1)

SA.pelvic:skin surface area of the pelvic ventrum, calculated using [SApelvic=1.15*(animal weight^0.559] (2)

Time: sampling time (hours)

Serum.conc: amount (micrograms) of chemical in 1 millilitre of serum (i.e., concentration in micrograms per millilitre)