In vivo percutaneous absorption data for chloramphenicol, formulated in 20% propylene glycol, in Rhinella marina

Brief description

In vivo percutaneous absorption kinetics of chloramphenicol in the terrestrial toad Rhinella marina.

Full description

The authors have previously produced a model of in vitro absorption in Rh. marina, and investigated the correlation between in vitro and in vivo percutaneous absorption for three model chemicals in this species. Such a model may find utility in design of treatment for disease in frogs. This model was used to select, formulate and predict the percutaneous absorption of chloramphenicol for the treatment of chytridiomycosis. This dataset provides in vivo absorption kinetics for chloramphenicol formulated in 20% propylene glycol, in the cane toad Rh. marina. 

Chloramphenicol was formulated individually at a dose of 250 mcg/ml in 20% v/v propylene glycol. 25 ml of the resultant solution was provided in a bath formulation to each animal, except for three animals used as controls that were exposed to 25 ml of 20%v/v propylene glycol solution only. This volume ensured exposure of solution to the entire ventral pelvis in each animal.  This study followed two phases: 1. Exposure (t=0-6 hours) and 2. Elimination (t=6-24 hours). During the exposure phase, animals were exposed to dosing solution. At t=6 hours, remaining animals were rinsed in purified water, returned to their individual enclosures, and monitored up to 24 hours. Sampling occurred throughout the exposure and elimination periods. Four animals were sacrificed at each sampling time and a cardiac blood sample taken. Prior to sacrifice, animals were rinsed in purified water to remove any remaining chemical solution. Rinsing and dosing solution were retained for analysis. Following extraction of serum, chemical quantification was undertaken using UHPLC. 

Data includes a spreadsheet (comma-separated values / .csv file) providing individual animal information (including animal ID, sex, snout-vent length and weight), rinse/dosing solution information (total volume and concentration of chloramphenicol in solution), and serum information (concentration of chloramphenicol in each serum sample). 

Column headings for the spreadsheet are explained below:

Chemical: chemical applied to the skin (caffeine/benzoic acid/ibuprofen)

Conc: concentration of chemical applied (microgram/ml)

AnimalID: individual animal identifier

Weight: frog weight in grams

SVL: animal snout-vent length (millimetres)

SA.total: total skin surface area calculated using [SAtotal=9.9*(animal weight^0.56)] (2)

SA.pelvic:skin surface area of the pelvic ventrum, calculated using [SApelvic=1.15*(animal weight^0.559] (3)

Time: sampling time (hours)

RinseBath.Vol: total volume of dosing solution PLUS rinse

Rinse.comc: amount (micrograms) of chloramphenicol in 1 millilitre of rinse (i.e., concentration in micrograms per ml)

Serum.conc: amount (micrograms) of chloramphenicol in 1 millilitre of serum (i.e., concentration in micrograms per millilitre)