Data

Illumina 16s amplicon sequencing data for in-situ Macquarie Island Mesocosm assessing the toxicity of residual hydrocarbons.

Australian Antarctic Data Centre
CRANE, SALLY L. ; FERRARI, BELINDA C ; HOSE, GRANT ; KING, CATHERINE K. ; ERRINGTON, INGRID ; GEORGE, SIMON CHRISTOPHER ; HOULAHAN, SARAH
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.4225/15/5949ac575daa7&rft.title=Illumina 16s amplicon sequencing data for in-situ Macquarie Island Mesocosm assessing the toxicity of residual hydrocarbons.&rft.identifier=10.4225/15/5949ac575daa7&rft.publisher=Australian Antarctic Data Centre&rft.description=This data set is Illumina 16s (bacterial) amplicon sequencing data for the Macquarie Island mesocosm ecotoxicology study. In-situ soil mesocosms (n=20) were set up on Macquarie Island in February 2013. Following a year’s equilibration, mesocosms were spiked in triplicate with a fuel mixture mimicking the composition of aged fuel spills on Macquarie Island, in addition to five solvent-only controls. Spiking concentrations range from 50mg/kg to 10000 mg/kg, all in triplicate, in addition to 5 solvent – only controls. Soils have been sampled from initial set up until April 2015, with a total of 2 pre-spike and 4 post-spike sample sets. DNA was extracted from soil in triplicate. Automated Ribosomal Intergenic Spacer analysis (ARISA) was conducted for both Fungal and Bacterial soil communities across all extractions (n = 270). ARISA results confirmed uniformity of replicate extractions. One replicate for each sample was selected for further work (n=90). Illumina sequencing was conducted with the 90 samples and these samples correspond with those also selected for microfluidic qPCR. See Thesis in publications section for more details. This data was processed in Mothur, using only the forward read. Missing samples are due to quality control, and duplicate samples are due to re-run in a subsequent Illumina submission. Three spreadsheets are included; - OTU abundance by sample, which is the key output document following processing in Mothur - OTU taxonomy classification, which identifies each OUT - Illumina with Enviro for Primer, which is a processed copy of the first document, together with key environmental data, for use in multivariate analysis and cross-referencing with microfluidic qPCR data . 'Environmental' tab provides additional data on samples, including TPH data and vegetation observations at one time point (expressed as percentage of coverage). Contact Sarah Houlahan for more information on chemical data. OTU = operational taxonomic unit. Sample Set/Action ### Date ### Weeks after spiking Establishment ### 17/01/2013 ### -45 weeks P1 ### 01/02/2013 ### -43 weeks P2 ### 30/10/2013 ### -3 weeks Spiking ### 19-25/11/2013 ### 0 weeks T1 ### 10/12/2013 ### 2 weeks T2 ### 30/03/2014 ### 18 weeks T3 ### 07/01/2015 ### 59 weeks T4 ### 24/03/2015 ### 69 weeks&rft.creator=CRANE, SALLY L. &rft.creator=FERRARI, BELINDA C &rft.creator=HOSE, GRANT &rft.creator=KING, CATHERINE K. &rft.creator=ERRINGTON, INGRID &rft.creator=GEORGE, SIMON CHRISTOPHER &rft.creator=HOULAHAN, SARAH &rft.date=2017&rft.coverage=northlimit=-54.49895152; southlimit=-54.49906754; westlimit=158.9391691; eastLimit=158.9392036; projection=WGS84&rft.coverage=northlimit=-54.49895152; southlimit=-54.49906754; westlimit=158.9391691; eastLimit=158.9392036; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4135_Hydrocarbon_Toxicity_amplicon when using these data.&rft_subject=environment&rft_subject=ECOTOXICOLOGY&rft_subject=EARTH SCIENCE&rft_subject=BIOSPHERE&rft_subject=ECOLOGICAL DYNAMICS&rft_subject=TOXICITY LEVELS&rft_subject=CONTAMINANT LEVELS/SPILLS&rft_subject=HUMAN DIMENSIONS&rft_subject=ENVIRONMENTAL IMPACTS&rft_subject=SOIL CHEMISTRY&rft_subject=LAND SURFACE&rft_subject=SOILS&rft_subject=BACTERIA/ARCHAEA&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=MESOCOSM&rft_subject=FUEL SPILL&rft_subject=DNA&rft_subject=ADS > Automated DNA Sequencer&rft_subject=FIELD INVESTIGATION&rft_subject=FIELD SURVEYS&rft_subject=LABORATORY&rft_subject=GEOGRAPHIC REGION > POLAR&rft_subject=OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4135_Hydrocarbon_Toxicity_amplicon when using these data.

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Brief description

This data set is Illumina 16s (bacterial) amplicon sequencing data for the Macquarie Island mesocosm ecotoxicology study. In-situ soil mesocosms (n=20) were set up on Macquarie Island in February 2013. Following a year’s equilibration, mesocosms were spiked in triplicate with a fuel mixture mimicking the composition of aged fuel spills on Macquarie Island, in addition to five solvent-only controls. Spiking concentrations range from 50mg/kg to 10000 mg/kg, all in triplicate, in addition to 5 solvent – only controls. Soils have been sampled from initial set up until April 2015, with a total of 2 pre-spike and 4 post-spike sample sets. DNA was extracted from soil in triplicate. Automated Ribosomal Intergenic Spacer analysis (ARISA) was conducted for both Fungal and Bacterial soil communities across all extractions (n = 270). ARISA results confirmed uniformity of replicate extractions. One replicate for each sample was selected for further work (n=90). Illumina sequencing was conducted with the 90 samples and these samples correspond with those also selected for microfluidic qPCR. See Thesis in publications section for more details. This data was processed in Mothur, using only the forward read. Missing samples are due to quality control, and duplicate samples are due to re-run in a subsequent Illumina submission. Three spreadsheets are included; - OTU abundance by sample, which is the key output document following processing in Mothur - OTU taxonomy classification, which identifies each OUT - Illumina with Enviro for Primer, which is a processed copy of the first document, together with key environmental data, for use in multivariate analysis and cross-referencing with microfluidic qPCR data . 'Environmental' tab provides additional data on samples, including TPH data and vegetation observations at one time point (expressed as percentage of coverage). Contact Sarah Houlahan for more information on chemical data. OTU = operational taxonomic unit. Sample Set/Action ### Date ### Weeks after spiking Establishment ### 17/01/2013 ### -45 weeks P1 ### 01/02/2013 ### -43 weeks P2 ### 30/10/2013 ### -3 weeks Spiking ### 19-25/11/2013 ### 0 weeks T1 ### 10/12/2013 ### 2 weeks T2 ### 30/03/2014 ### 18 weeks T3 ### 07/01/2015 ### 59 weeks T4 ### 24/03/2015 ### 69 weeks

Issued: 2017-06-20

Data time period: 2013-01-17 to 2015-03-24

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158.9392,-54.49895 158.9392,-54.49907 158.93917,-54.49907 158.93917,-54.49895 158.9392,-54.49895

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text: northlimit=-54.49895152; southlimit=-54.49906754; westlimit=158.9391691; eastLimit=158.9392036; projection=WGS84

Subjects
ADS > Automated DNA Sequencer | BACTERIA/ARCHAEA | BIOLOGICAL CLASSIFICATION | BIOSPHERE | CONTAMINANT LEVELS/SPILLS | DNA | EARTH SCIENCE | ECOLOGICAL DYNAMICS | ECOTOXICOLOGY | ENVIRONMENTAL IMPACTS | FIELD INVESTIGATION | FIELD SURVEYS | FUEL SPILL | GEOGRAPHIC REGION > POLAR | HUMAN DIMENSIONS | LABORATORY | LAND SURFACE | MESOCOSM | OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND | SOIL CHEMISTRY | SOILS | TOXICITY LEVELS | environment |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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