Data

Heterologous Expression of Carboxysomes in Plants

Also known as: Carboxysomes in Plants
The Australian National University
Long, Benedict ; Hee, Wei Yih ; Price, Dean
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25911/5b4edb4bada74&rft.title=Heterologous Expression of Carboxysomes in Plants &rft.identifier=10.25911/5b4edb4bada74&rft.publisher=The Australian National University Data Commons&rft.description=The data held within this document are the raw data collected for experiments on the heterologous expression of alpha-carboxysomes from the marine cyanobacterium Cyanobium marinum PCC7001 in chloroplasts of Nicotiana tabacum. Genes were introduced by biolistic bombardment to replace the endogenous Rubisco large subunit gene with the Rubisco large and small subunit gens from Cyanobium, or these genes in concert with the carboxysomal protein genes csoS1A and csoS2. Data presented here include; raw data images of Southern blots confirming transgene insertion, raw images of transmission electron micrographs of transgenic plant chloroplasts and isolated carboxysomes, SDS-PAGE and immunoblot images, raw data for Nanosight N300 particle size analysis (including video data), gas exchange data for wild-type and transgenic plants, membrane inlet mass spectrometric measurement data of CO2 utilization by leaf discs of wild-type and transgenic plants, and growth data of each plant type. Nanosight N300 data can be reanalyzed using the appropriate proprietary software. Raw numerical data are supplied as .csv files for analysis by standard spreadsheet and graphing software. Images are supplied as .tif files.This dataset reports the first successful expression of encapsulated Rubisco enzymes in simplified carboxysomes within tobacco chloroplasts. This work forms the basis of attempts to generate functional CO2 concentrating mechanisms within C3 plant chloroplasts to enhance photosynthesis and yield.&rft.creator=Long, Benedict &rft.creator=Hee, Wei Yih &rft.creator=Price, Dean &rft.date=2018&rft_rights= http://legaloffice.weblogs.anu.edu.au/content/copyright/&rft_subject=Plant Cell and Molecular Biology&rft_subject=BIOLOGICAL SCIENCES&rft_subject=PLANT BIOLOGY&rft_subject=Microbial Genetics&rft_subject=MICROBIOLOGY&rft_subject=Synthetic Biology&rft_subject=BIOCHEMISTRY AND CELL BIOLOGY&rft_subject=Biochemistry and Cell Biology not elsewhere classified&rft.type=dataset&rft.language=English Access the data
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Research School of Biology, Linnaeus Way, The Australian National University, 2601 ACT

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Full description

The data held within this document are the raw data collected for experiments on the heterologous expression of alpha-carboxysomes from the marine cyanobacterium Cyanobium marinum PCC7001 in chloroplasts of Nicotiana tabacum. Genes were introduced by biolistic bombardment to replace the endogenous Rubisco large subunit gene with the Rubisco large and small subunit gens from Cyanobium, or these genes in concert with the carboxysomal protein genes csoS1A and csoS2. Data presented here include; raw data images of Southern blots confirming transgene insertion, raw images of transmission electron micrographs of transgenic plant chloroplasts and isolated carboxysomes, SDS-PAGE and immunoblot images, raw data for Nanosight N300 particle size analysis (including video data), gas exchange data for wild-type and transgenic plants, membrane inlet mass spectrometric measurement data of CO2 utilization by leaf discs of wild-type and transgenic plants, and growth data of each plant type. Nanosight N300 data can be reanalyzed using the appropriate proprietary software. Raw numerical data are supplied as .csv files for analysis by standard spreadsheet and graphing software. Images are supplied as .tif files.

Notes

5.3 GB.

Significance statement

This dataset reports the first successful expression of encapsulated Rubisco enzymes in simplified carboxysomes within tobacco chloroplasts. This work forms the basis of attempts to generate functional CO2 concentrating mechanisms within C3 plant chloroplasts to enhance photosynthesis and yield.

Created: 2017

Data time period: 2015-06-06 to 2018-03-06

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Subjects
Biochemistry and Cell Biology | Biological Sciences | Biochemistry and Cell Biology Not Elsewhere Classified | Microbiology | Microbial Genetics | Plant Biology | Plant Cell and Molecular Biology | Synthetic Biology |

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Identifiers
ACN 633 798 857