Data

Growth rate and Fast Repetition Rate fluorometry (FRRf) of phytoplankton

University of Tasmania, Australia
Guo, Jiaying
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25959/1Z63-7555&rft.title=Growth rate and Fast Repetition Rate fluorometry (FRRf) of phytoplankton&rft.identifier=10.25959/1Z63-7555&rft.description=Ocean alkalinity enhancement (OAE) is a promising carbon removal method, but it may cause a significant perturbation of the ocean with trace metals such as Nickel (Ni). This study tested the effect of increasing Ni concentrations on phytoplankton growth and photosynthesis. The data were the growth rates of 11 phytoplankton species under different Ni concentrations (Master thesis project). The growth rates were calculated using daily fluorescence signal values measured by the fluorometer. Fv/Fm and SigmaPSII data were measured using Fast repetition rate fluorometry (FRRf). The growth rate and photo-physiological response of phytoplankton was analysed using generalised additive models (GAMs) and plotted in RStudio (R packages “mgcv” and “ggplot2”).Phytoplankton cultures were kept in tubes and grown at 17°C. The light was provided on a 14-to-10-hour daily cycle (cool white fluorescence light) where light intensities were 58 μmol photons m-2 s-1 (14 hours) and 23 μmol photons m-2 s-1 (10 hours). The fluorescence of these cultures was measured daily using a fluorometer and values during the exponential growth phase were ln-transferred for growth rate calculation. Fv/Fm and SigmaPSII data were measured using Fast repetition rate fluorometry (FRRf). The growth rate and photo-physiological response of phytoplankton were analysed using generalised additive models (GAMs) and plotted in RStudio (R packages “mgcv” and “ggplot2”).&rft.creator=Guo, Jiaying &rft.date=2015&rft_rights=Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0&rft_subject=biota&rft_subject=MICROALGAE&rft_subject=EARTH SCIENCE&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=PLANTS&rft_subject=CYANOBACTERIA (BLUE-GREEN ALGAE)&rft_subject=BACTERIA/ARCHAEA&rft_subject=TRACE METALS&rft_subject=TERRESTRIAL HYDROSPHERE&rft_subject=WATER QUALITY/WATER CHEMISTRY&rft_subject=Growth rate&rft_subject=total dissolved nickel concentrations&rft_subject=pNi&rft_subject=free nickel ion concentrations&rft_subject=negtively log10-transforred free nickel ion concentrations&rft_subject=Minimum ST-ChlF in the dark-regulated state&rft_subject=Maximum ST-ChlF in the dark-regulated state&rft_subject=the maximum quantum yield&rft_subject=the functional absorption cross-section of photosystem II (σPSII)&rft_subject=re- opening of closed reaction center II (RCII)&rft_subject=Estimated degrees of freedom for the model terms&rft_subject=estimated residual degrees of freedom&rft_subject=Simply a ratio of two variances&rft_subject=number of basis functions&rft_subject=k-index&rft_subject=Adjusted r squred&rft_subject=95% confidence interval&rft_subject=Deviance explained&rft_subject=Biological Oceanography&rft_subject=EARTH SCIENCES&rft_subject=OCEANOGRAPHY&rft.type=dataset&rft.language=English Access the data

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Brief description

Ocean alkalinity enhancement (OAE) is a promising carbon removal method, but it may cause a significant perturbation of the ocean with trace metals such as Nickel (Ni). This study tested the effect of increasing Ni concentrations on phytoplankton growth and photosynthesis. The data were the growth rates of 11 phytoplankton species under different Ni concentrations (Master thesis project). The growth rates were calculated using daily fluorescence signal values measured by the fluorometer. Fv/Fm and SigmaPSII data were measured using Fast repetition rate fluorometry (FRRf). The growth rate and photo-physiological response of phytoplankton was analysed using generalised additive models (GAMs) and plotted in RStudio (R packages “mgcv” and “ggplot2”).

Lineage

Phytoplankton cultures were kept in tubes and grown at 17°C. The light was provided on a 14-to-10-hour daily cycle (cool white fluorescence light) where light intensities were 58 μmol photons m-2 s-1 (14 hours) and 23 μmol photons m-2 s-1 (10 hours). The fluorescence of these cultures was measured daily using a fluorometer and values during the exponential growth phase were ln-transferred for growth rate calculation. Fv/Fm and SigmaPSII data were measured using Fast repetition rate fluorometry (FRRf). The growth rate and photo-physiological response of phytoplankton were analysed using generalised additive models (GAMs) and plotted in RStudio (R packages “mgcv” and “ggplot2”).

Notes

Credit
This study was funded by the Australian Research Council by Future Fellowship FT200100846 awarded to Lennart Bach.

Data time period: 2021-03-01 to 2021-08-31

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Other Information
(DATA ACCESS - growth rate and fluorometry data [direct file download])

uri : https://data.imas.utas.edu.au/attachments/5aa2f15d-cac9-4b6c-9955-ddcfd61f8534/

Identifiers