Data

Great Barrier Reef HPLC Pigment and Ocean Colour Cruise October 1999

Australian Ocean Data Network
CSIRO O&A, Information & Data Centre (Point of contact) Clementson, Lesley (Point of contact) Lenton, Andrew (Associated with)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/4f41786b-4d56-41b8-b5b3-5cd553aa29a3&rft.title=Great Barrier Reef HPLC Pigment and Ocean Colour Cruise October 1999&rft.identifier=Anzlic Identifier: ANZCW0306006425&rft.publisher=Australian Ocean Data Network&rft.description=This record contains data collected on the Lady Basten (AIMS cruise code LB2401/99) between 11 and 18 October 1999 from waters in the Great Barrier Reef near Rib Reef, Pandora Reef and Orpheus Island. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and the absorption coefficient for dissolved (CDOM), particulate and detrital or non-algal components of the water column.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the RV Lady Basten. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis: 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption: 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer.&rft.creator=Anonymous&rft.date=2004&rft.coverage=westlimit=145; southlimit=-18; eastlimit=147; northlimit=-17&rft.coverage=westlimit=145; southlimit=-18; eastlimit=147; northlimit=-17&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=CHLOROPHYLL&rft_subject=EARTH SCIENCE&rft_subject=OCEANS&rft_subject=OCEAN CHEMISTRY&rft_subject=PIGMENTS&rft_subject=ABSORPTION&rft_subject=OCEAN OPTICS&rft_subject=GELBSTOFF&rft_subject=OCEAN COLOR&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Marine Features (Australia) | Great Barrier Reef, QLD&rft_subject=WfO Biogeochemistry&rft_subject=Ocean Colour 1997-2000&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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This record contains data collected on the Lady Basten (AIMS cruise code LB2401/99) between 11 and 18 October 1999 from waters in the Great Barrier Reef near Rib Reef, Pandora Reef and Orpheus Island. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and the absorption coefficient for dissolved (CDOM), particulate and detrital or non-algal components of the water column.

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Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the RV Lady Basten. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis: 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption: 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer.

Notes

Credit
CSIRO Earth Observation Centre - funding
Credit
Lesley Clementson
Credit
John Parslow
Credit
Tiit Kutser
Credit
William Skirving (AIMS)
Credit
Terry Done (AIMS)
Credit
Ian Millar (AIMS) and Mary Wakeford (AIMS)

Data time period: 1999-10-11 to 1999-10-18

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147,-17 147,-18 145,-18 145,-17 147,-17

146,-17.5

text: westlimit=145; southlimit=-18; eastlimit=147; northlimit=-17

Subjects
Absorption | Chlorophyll | CTD | CTDs (Conductivity-Temperature-Depth Profilers) | EARTH SCIENCE | Fluorometers | Gelbstoff | HPLC (High Performance Liquid Chromatography) | Marine Features (Australia) | Great Barrier Reef, QLD | Niskin Bottles | OCEAN CHEMISTRY | Ocean Color | OCEAN OPTICS | OCEANS | Ocean Colour 1997-2000 | Pigments | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

uri : https://creativecommons.org/licenses/by/4.0/

Identifiers
  • global : 4f41786b-4d56-41b8-b5b3-5cd553aa29a3
  • Local : Marlin Record Number: 6425
  • Local : Anzlic Identifier: ANZCW0306006425
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