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Brief description
This record contains data collected on the charter vessel RV Lady Basten between 23 and 28 August 2005 in the Great Barrier Reef. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate, the absorption coefficient for dissolved (aCDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column. 20 stations were sampled. The data was used primarily to validate ocean colour sensors MERIS, MODIS and SeaWIFs and the SST sensor AATSR. Samples were collected and analysed for pigments, total suspended solids (TSS) and dissolved and particulate absorption coefficients. CLW deployed instruments RAMSES, ac-9, Hydroscat and Hydrorad.Lineage
Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the RV Lady Basten and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube.
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.
Notes
CreditIan Barton
Credit
Lesley Clementson
Lesley Clementson
Credit
Janet Anstee (CLW)
Janet Anstee (CLW)
Credit
Paul Daniel(CLW)
Paul Daniel(CLW)
Credit
Vittorio Brando(CLW)
Vittorio Brando(CLW)
Data time period: 2005-08-23 to 2005-08-28
Subjects
Chlorophyll |
CTD |
CTDs (Conductivity-Temperature-Depth Profilers) |
EARTH SCIENCE |
Fluorometers |
HPLC (High Performance Liquid Chromatography) |
Marine Features (Australia) | Great Barrier Reef Central Section, QLD |
Niskin Bottles |
OCEAN CHEMISTRY |
Ocean Color |
OCEAN OPTICS |
OCEANS |
Pigments |
Practical salinity of the water body |
Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it |
Pressure (measured variable) in the water body exerted by overlying sea water only |
Remote Sensing 2004-2007 |
Suspended Solids |
Ship: Lady Basten |
Spectrophotometers |
Temperature of the water body |
WfO Biogeochemistry |
oceans |
research vessel |
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Other Information
Data also available via IMOS and AODN portal (Data Link)
uri :
https://imos.aodn.org.au/webportal/
Donwload LC_IMOS_SRS_BioOptical_GBRaug2005_TSS.zip (.csv, .xls, .nc) (Data Link)
Download LC_IMOS_SRS_BioOptical_GBRaug2005_absorption.zip (.csv, .xls, .nc) (Data Link)
Download LC_IMOS_SRS_BioOptical_GBRaug2005_pigments.zip (.csv, .xls, .nc) (Data Link)
This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)
Identifiers
- global : d3c5d7ba-b861-72d5-e043-08114f8c2cf2
- Local : Marlin Record Number: 14181
- Local : Anzlic Identifier: ANZCW0306014181
