Data

Great Barrier Reef , June 2002 , HPLC Pigment and Ocean Colour Data (AIMS cruise code LB0202)

Australian Ocean Data Network
CSIRO O&A, Information & Data Centre (Point of contact) Clementson, Lesley (Point of contact) Lenton, Andrew (Associated with)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/4acd08f0-6219-407c-b00a-521e53158e60&rft.title=Great Barrier Reef , June 2002 , HPLC Pigment and Ocean Colour Data (AIMS cruise code LB0202)&rft.identifier=Anzlic Identifier: ANZCW0306006376&rft.publisher=Australian Ocean Data Network&rft.description=This record contains data collected on the AIMS vessel RV Lady Basten between 5 and 13 June 2002. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate. 9 stations were sampled. This cruise was used primarily to validate ocean colour sensors MERIS, MODIS and SeaWIFS and the SST sensor AATSR. Samples were collected for pigments, total suspended solids (TSS) and absorption coefficient- dissolved and particulate.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the Lady Basten. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped&rft.creator=Anonymous&rft.date=2004&rft.coverage=westlimit=146; southlimit=-21; eastlimit=150.5; northlimit=-18&rft.coverage=westlimit=146; southlimit=-21; eastlimit=150.5; northlimit=-18&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=CHLOROPHYLL&rft_subject=EARTH SCIENCE&rft_subject=OCEANS&rft_subject=OCEAN CHEMISTRY&rft_subject=PIGMENTS&rft_subject=OCEAN COLOR&rft_subject=OCEAN OPTICS&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Marine Features (Australia) | Great Barrier Reef Central Section, QLD&rft_subject=WfO Biogeochemistry&rft_subject=Remote Sensing 2000-2003&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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This record contains data collected on the AIMS vessel RV Lady Basten between 5 and 13 June 2002. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate. 9 stations were sampled. This cruise was used primarily to validate ocean colour sensors MERIS, MODIS and SeaWIFS and the SST sensor AATSR. Samples were collected for pigments, total suspended solids (TSS) and absorption coefficient- dissolved and particulate.

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Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the Lady Basten. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped

Notes

Credit
Lesley Clementson
Credit
Dave Terhell
Credit
Ian Barton

Data time period: 2002-06-05 to 2002-06-10

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150.5,-18 150.5,-21 146,-21 146,-18 150.5,-18

148.25,-19.5

Subjects
Chlorophyll | CTD | CTDs (Conductivity-Temperature-Depth Profilers) | EARTH SCIENCE | Fluorometers | HPLC (High Performance Liquid Chromatography) | Marine Features (Australia) | Great Barrier Reef Central Section, QLD | Niskin Bottles | OCEAN CHEMISTRY | Ocean Color | OCEAN OPTICS | OCEANS | Pigments | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Remote Sensing 2000-2003 | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

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Identifiers
  • global : 4acd08f0-6219-407c-b00a-521e53158e60
  • Local : Marlin Record Number: 6376
  • Local : Anzlic Identifier: ANZCW0306006376
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