Brief description
This record contains data generated from samples collected annually in Gladstone Harbour, from 2012-2014. These samples were taken as part of the GISERA marine environmental research program. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments. Between 9 and 42 stations were sampled each year.Lineage
Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were collected, and then stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube.
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.
Notes
CreditRussell Babcock (O&A)
Credit
Lesley Clementson (O&A)
Lesley Clementson (O&A)
Credit
Mark Baird (O&A)
Mark Baird (O&A)
Credit
Kadija Oubelkheir (L&W)
Kadija Oubelkheir (L&W)
Credit
Gary Fry (O&A)
Gary Fry (O&A)
Data time period: 2012-11-20 to 2014-10-24
text: westlimit=151.13; southlimit=-23.676; eastlimit=151.372; northlimit=-23.909; projection=WGS 84 (EPSG:4326)
Subjects
Chlorophyll |
CTD |
CTDs (Conductivity-Temperature-Depth Profilers) |
Coastal Cities / Towns (Australia) | Gladstone, QLD |
EARTH SCIENCE |
Filtration Systems |
Fluorometers |
Gladstone Harbour Model |
HPLC (High Performance Liquid Chromatography) |
Niskin Bottles |
OCEAN CHEMISTRY |
Ocean Color |
OCEAN OPTICS |
OCEANS |
Pigments |
Practical salinity of the water body |
Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it |
Pressure (measured variable) in the water body exerted by overlying sea water only |
Suspended Solids |
Spectrophotometers |
Temperature of the water body |
WfO Biogeochemistry |
oceans |
research vessel |
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Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))
uri :
https://aesop.csiro.au/
Data also available via IMOS and AODN portal (IMOS and AODN portal)
Identifiers
- global : b20a3908-7800-47ea-b02b-f7cae6e4f385
