Data

Genetic diversity of the sea cucumber, Holothuria scabra in New Caledonia, Australia (Northern Territory) and Bali

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Australian Institute of Marine Science (Owned by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.gov.au/dataset/0614d1ac-2639-43d0-aaaf-9b8b6cec817b&rft.title=Genetic diversity of the sea cucumber, Holothuria scabra in New Caledonia, Australia (Northern Territory) and Bali&rft.identifier=genetic-diversity-of-the-sea-cucumber-holothuria-scabra-in-new-caledonia-australia-northern-ter&rft.publisher=data.gov.au&rft.description=Point of truth URL of this metadata record - http://gcmd.nasa.gov/Resources/valids/archives/keyword_list.html - Within-region genetic heterogeneity of Holothuria scabra populations in New Caledonia was examined through allozyme electrophoresis. Five locations, each separated by 60-130 km, were selected along the coast of New Caledonia and 2 replicate sites (8-23 km apart) were chosen within 4 of these locations on the west coast of the island. Only one site was selected on the northeast coast. Between 14 and 34 adult Holothuria scabra were collected from each site. A short section of the upper intestine (5-10 cm), without sediments, was dissected from each animal and stored in liquid nitrogen until analysed. \n\nHolothuria scabra samples from Bali (n=90) and Knocker Bay, Australia (n=47), and existing data from the western Pacific: Solomon Islands; Torres Strait; and the Queensland Coast (Upstart Bay and Hervey Bay) were compared with the data from New Caledonia to investigate larval connectivity over a wider geographic scale. \n\nSeven polymorphic enzyme loci were surveyed using allozyme electrophoresis: GPI, HK, MDH, PEP-1, PEP-2, PEP-3 and PGM. Basic analyses of genetic variability (allele frequencies, number of polymorphic loci, heterozygosity) were carried out. The allelic richness for each population was calculated. F statistics were calculated and genetic distance analyses, cluster analyses and Mantel tests were performed.&rft.creator=Australian Institute of Marine Science&rft.date=2023&rft.coverage=163.5,-22.5 167.5,-22.5 167.5,-19.6 163.5,-19.6 163.5,-22.5&rft.coverage=163.5,-22.5 167.5,-22.5 167.5,-19.6 163.5,-19.6 163.5,-22.5&rft.coverage=true&rft_rights=Other&rft_subject=Aquatic Sciences&rft_subject=Aspidochirotida&rft_subject=Biological Classification&rft_subject=Biosphere&rft_subject=Coastal Processes&rft_subject=Coral Reefs&rft_subject=Echinoderms&rft_subject=Ecological Dynamics&rft_subject=Electrophoresis&rft_subject=Fisheries&rft_subject=Holothuria scabra&rft_subject=Holothuroidea&rft_subject=Invertebrate fisheries&rft_subject=Oceans&rft_subject=Population genetics&rft_subject=Restocking&rft_subject=Sandfish&rft_subject=Sea cucumber&rft_subject=Trepang&rft.type=dataset&rft.language=English Access the data

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Brief description

Within-region genetic heterogeneity of Holothuria scabra populations in New Caledonia was examined through allozyme electrophoresis. Five locations, each separated by 60-130 km, were selected along the coast of New Caledonia and 2 replicate sites (8-23 km apart) were chosen within 4 of these locations on the west coast of the island. Only one site was selected on the northeast coast. Between 14 and 34 adult Holothuria scabra were collected from each site. A short section of the upper intestine (5-10 cm), without sediments, was dissected from each animal and stored in liquid nitrogen until analysed. \n\nHolothuria scabra samples from Bali (n=90) and Knocker Bay, Australia (n=47), and existing data from the western Pacific: Solomon Islands; Torres Strait; and the Queensland Coast (Upstart Bay and Hervey Bay) were compared with the data from New Caledonia to investigate larval connectivity over a wider geographic scale. \n\nSeven polymorphic enzyme loci were surveyed using allozyme electrophoresis: GPI, HK, MDH, PEP-1, PEP-2, PEP-3 and PGM. Basic analyses of genetic variability (allele frequencies, number of polymorphic loci, heterozygosity) were carried out. The allelic richness for each population was calculated. F statistics were calculated and genetic distance analyses, cluster analyses and Mantel tests were performed.

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Point of truth URL of this metadata record -
http://gcmd.nasa.gov/Resources/valids/archives/keyword_list.html -

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163.5,-22.5 167.5,-22.5 167.5,-19.6 163.5,-19.6 163.5,-22.5

165.5,-21.05

163.5,-22.5 167.5,-22.5 167.5,-19.6 163.5,-19.6 163.5,-22.5

165.5,-21.05

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