Full description
A GC-MS is composed of three basics building blocks:1. A means of introducing the sample via an injection port.
2. The separation of mixtures of compounds by partitioning them between a gas phase (typically helium) and an activated solid phase attached to a silica capillary column. The difference in the chemical properties between different molecules in a mixture and their relative affinity for the stationary phase of the column will promote separation of the molecules as the sample travels the length of the column. The molecules are retained by the column and then elute (come off) from the column at different times (called the retention time).
3. As the helium gas phase containing the molecules passes through the mass spectrometer, they are ionised into fragments and detected based on their mass charge ratio. The fragment pattern can be match to an internal library to provide the absolute identification of a compound.
The true power of the GC-MS is when the information of the retention time and mass fragment patterns are combined to allowed the identification of compounds in extremely complex samples.
To cite this equipment/component: Research Infrastructure Centres. (2026). Gas Chromatography Mass Spectrometry (GCMS) - Agilent GCMS 8890 GC + 5977C MSD. The University of Western Australia. https://doi.org/10.26182/R09C-2W60
Subjects
Electron ionisation (EI) |
Fatty acid methyl ester analysis in multiple sample types |
Gas chromatography |
Mass spectrometry |
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Identifiers
- DOI : 10.26182/R09C-2W60
- global : d3db73c0-6d4d-4c15-b4a7-a8b52eb8a263
