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Data from: Using SANS with contrast-matched lipid bicontinuous cubic phases to determine the location of encapsulated peptides, proteins, and other biomolecules

RMIT University, Australia
Calum John Drummond (Aggregated by) Dr Charlotte Conn (Associated with, Aggregated by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://figshare.com/articles/Using_SANS_with_Contrast-Matched_Lipid_Bicontinuous_Cubic_Phases_To_Determine_the_Location_of_Encapsulated_Peptides_Proteins_and_Other_Biomolecules/3486311&rft.title=Data from: Using SANS with contrast-matched lipid bicontinuous cubic phases to determine the location of encapsulated peptides, proteins, and other biomolecules&rft.identifier=3e084dd0543817642950211413249ba2&rft.publisher=RMIT University, Australia&rft.description=Attached file provides supplementary data for linked article. An understanding of the location of peptides, proteins, and other biomolecules within the bicontinuous cubic phase is crucial for understanding and evolving biological and biomedical applications of these hybrid biomolecule lipid materials, including during in meso crystallization and drug delivery. While theoretical modeling has indicated that proteins and additive lipids might phase separate locally and adopt a preferred location in the cubic phase, this has never been experimentally confirmed. We have demonstrated that perfectly contrast-matched cubic phases in D2O can be studied using small-angle neutron scattering by mixing fully deuterated and hydrogenated lipid at an appropriate ratio. The model transmembrane peptide WALP21 showed no preferential location in the membrane of the diamond cubic phase of phytanoyl monoethanolamide and was not incorporated in the gyroid cubic phase. While deuteration had a small effect on the phase behavior of the cubic phase forming lipids, the changes did not significantly affect our results.&rft.creator=Calum John Drummond&rft.creator=Dr Charlotte Conn&rft.date=2018&rft.relation=http://dx.doi.org/10.1021/acs.jpclett.6b01173&rft_rights=Further information about rights and usage of ACS publications and supplementary data can be found here: http://pubs.acs.org/page/copyright/permissions.html.&rft_rights=CC BY-NC: Attribution-Noncommercial 3.0 AU http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=Biological materials&rft_subject=Peptides Bicontinuous cubic phase &rft_subject=Bicontinuous cubic phasis&rft_subject=Biomedical applications&rft_subject=Cubic phasis&rft_subject=Deuterations &rft_subject=Diamond cubic phase&rft_subject=Theoretical modeling&rft_subject=Transmembraneslts&rft_subject=Biomolecules&rft_subject=Deuterium&rft_subject=Hybrid materials&rft_subject=Lipids&rft_subject=Location &rft_subject=Medical applications&rft_subject=Molecular biology&rft_subject=Neutron scattering&rft_subject=Physical Chemistry not elsewhere classified&rft_subject=CHEMICAL SCIENCES&rft_subject=PHYSICAL CHEMISTRY (INCL. STRUCTURAL)&rft.type=dataset&rft.language=English Access the data
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http://creativecommons.org/licenses/by-nc/3.0/au

Further information about rights and usage of ACS publications and supplementary data can be found here: http://pubs.acs.org/page/copyright/permissions.html.

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Attached file provides supplementary data for linked article. An understanding of the location of peptides, proteins, and other biomolecules within the bicontinuous cubic phase is crucial for understanding and evolving biological and biomedical applications of these hybrid biomolecule lipid materials, including during in meso crystallization and drug delivery. While theoretical modeling has indicated that proteins and additive lipids might phase separate locally and adopt a preferred location in the cubic phase, this has never been experimentally confirmed. We have demonstrated that perfectly contrast-matched cubic phases in D2O can be studied using small-angle neutron scattering by mixing fully deuterated and hydrogenated lipid at an appropriate ratio. The model transmembrane peptide WALP21 showed no preferential location in the membrane of the diamond cubic phase of phytanoyl monoethanolamide and was not incorporated in the gyroid cubic phase. While deuteration had a small effect on the phase behavior of the cubic phase forming lipids, the changes did not significantly affect our results.

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Bicontinuous cubic phasis | Biological materials | Biomedical applications | Biomolecules | Chemical Sciences | Cubic phasis | Deuterations | Deuterium | Diamond cubic phase | Hybrid materials | Lipids | Location | Medical applications | Molecular biology | Neutron scattering | Physical Chemistry (Incl. Structural) | Peptides Bicontinuous cubic phase | Physical Chemistry Not Elsewhere Classified | Theoretical modeling | Transmembraneslts |

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