Data

Data from: In meso crystallization: compatibility of different lipid bicontinuous cubic mesophases with the cubic crystallization screen in aqueous solution

RMIT University, Australia
Calum John Drummond (Aggregated by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://figshare.com/articles/In_Meso_Crystallization_Compatibility_of_Different_Lipid_Bicontinuous_Cubic_Mesophases_with_the_Cubic_Crystallization_Screen_in_Aqueous_Solution/2310067&rft.title=Data from: In meso crystallization: compatibility of different lipid bicontinuous cubic mesophases with the cubic crystallization screen in aqueous solution&rft.identifier=d8323f514bac807facdd10519b02fbe1&rft.publisher=RMIT University, Australia&rft.description=Attached file provides supplementary data for linked article. In meso crystallization uses bicontinuous cubic lipidic mesophases as matrices for the crystallization of membrane proteins. In this work, we look at the impact of a screen specifically marketed as compatible with the cubic mesophase, the Cubic crystallization screen (Emerald BioSystems), on the cubic mesophases formed by three different lipids: monoolein, monopalmitolein, and phytantriol. The Cubic screen was found to be compatible with cubic mesophase retention under most crystallization conditions for all three lipids studied. The effect of the individual components comprising the multicomponent screen was deconvoluted in two ways. Initially, the effect of specific poly(ethylene glycol) (PEG) and salt components on the cubic mesophase was determined using small-angle X-ray scattering (SAXS). The effect of high-molecular-weight (M w) PEG was shown to dominate the phase behavior within the screen. The effect of additional salts present within the screen becomes important for low Mw PEG molecules. Finally, a recently developed multiple linear-regression modeling method was shown to deconvolute the effect of individual components within the screen effectively.&rft.creator=Calum John Drummond&rft.date=2018&rft.relation=https://dx.doi.org/10.1021/cg4018954&rft_rights=Further information about rights and usage of ACS publications and supplementary data can be found here: http://pubs.acs.org/page/copyright/permissions.html.&rft_rights=CC BY-NC: Attribution-Noncommercial 3.0 AU http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=Biological membranes&rft_subject=Multiple linear-regression models&rft_subject=Small angle X-ray scattering&rft_subject=Thermotropic liquid crystals&rft_subject=Lipids&rft_subject=Polyethylene glycols&rft_subject=Crystallization conditions&rft_subject=Cubic mesophases&rft_subject=Effect of individual components&rft_subject=High molecular weight&rft_subject=Individual components&rft_subject=Membrane proteins&rft_subject=Inorganic Chemistry not elsewhere classified&rft_subject=CHEMICAL SCIENCES&rft_subject=INORGANIC CHEMISTRY&rft.type=dataset&rft.language=English Access the data
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Attached file provides supplementary data for linked article. In meso crystallization uses bicontinuous cubic lipidic mesophases as matrices for the crystallization of membrane proteins. In this work, we look at the impact of a screen specifically marketed as compatible with the cubic mesophase, the Cubic crystallization screen (Emerald BioSystems), on the cubic mesophases formed by three different lipids: monoolein, monopalmitolein, and phytantriol. The Cubic screen was found to be compatible with cubic mesophase retention under most crystallization conditions for all three lipids studied. The effect of the individual components comprising the multicomponent screen was deconvoluted in two ways. Initially, the effect of specific poly(ethylene glycol) (PEG) and salt components on the cubic mesophase was determined using small-angle X-ray scattering (SAXS). The effect of high-molecular-weight (M w) PEG was shown to dominate the phase behavior within the screen. The effect of additional salts present within the screen becomes important for low Mw PEG molecules. Finally, a recently developed multiple linear-regression modeling method was shown to deconvolute the effect of individual components within the screen effectively.

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Subjects
Biological membranes | Chemical Sciences | Crystallization conditions | Cubic mesophases | Effect of individual components | High molecular weight | Inorganic Chemistry | Individual components | Inorganic Chemistry Not Elsewhere Classified | Lipids | Membrane proteins | Multiple linear-regression models | Polyethylene glycols | Small angle X-ray scattering | Thermotropic liquid crystals |

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  • Local : d8323f514bac807facdd10519b02fbe1
ACN 633 798 857