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Data from: Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity

The University of Western Australia
Whitehead, Michael R. ; Catullo, Renee A. ; Ruibal, Monica ; Dixon, Kingsley W. ; Peakall, Rod ; Linde, Celeste C.
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.5061/dryad.2hf40&rft.title=Data from: Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity&rft.identifier=10.5061/dryad.2hf40&rft.publisher=Dryad Digital Repository&rft.description=The increasing availability of DNA sequence data enables exciting new opportunities for fungal ecology. However, it amplifies the challenge of how to objectively classify the diversity of fungal sequences into meaningful units, often in the absence of morphological characters. Here, we test the utility of modern multilocus Bayesian coalescent-based methods for delimiting cryptic fungal diversity in the orchid mycorrhiza morphospecies Serendipita vermifera. We obtained 147 fungal isolates from Caladenia, a speciose clade of Australian orchids known to associate with Serendipita fungi. DNA sequence data for 7 nuclear and mtDNA loci were used to erect competing species hypotheses by clustering isolates based on: (a) ITS sequence divergence, (b) Bayesian admixture analysis, and (c) mtDNA variation. We implemented two coalescent-based Bayesian methods to determine which species hypothesis best fitted our data. Both methods found strong support for eight species of Serendipita among our isolates, supporting species boundaries reflected in ITS divergence. Patterns of host plant association showed evidence for both generalist and specialist associations within the host genus Caladenia. Our findings demonstrate the utility of Bayesian species delimitation methods and suggest that wider application of these techniques will readily uncover new species in other cryptic fungal lineages.,FUNECO_605_SerendipitaATP6Sequence data: locus ATP6FUNECO_605_SerendipitaC11488Sequence data: locus C11488FUNECO_605_SerendipitaC11804Sequence data: locus C11804FUNECO_605_SerendipitaC16699Sequence data: locus C16699FUNECO_605_SerendipitaC28586Sequence data: locus C28586FUNECO_605_SerendipitaITS1-4Sequence data: locus ITSFUNECO_605_SerendipitaLSUSequence data: locus LSUFUNECO_605_StarbeastXMLStarbeast XML files for four species delimitation hypotheses: T=2, T=3, T=7, and T=8,&rft.creator=Whitehead, Michael R. &rft.creator=Catullo, Renee A. &rft.creator=Ruibal, Monica &rft.creator=Dixon, Kingsley W. &rft.creator=Peakall, Rod &rft.creator=Linde, Celeste C. &rft.date=2018&rft.relation=http://research-repository.uwa.edu.au/en/publications/98a298fa-397b-47da-994f-fc25f650fae1&rft.type=dataset&rft.language=English Access the data

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The increasing availability of DNA sequence data enables exciting new opportunities for fungal ecology. However, it amplifies the challenge of how to objectively classify the diversity of fungal sequences into meaningful units, often in the absence of morphological characters. Here, we test the utility of modern multilocus Bayesian coalescent-based methods for delimiting cryptic fungal diversity in the orchid mycorrhiza morphospecies Serendipita vermifera. We obtained 147 fungal isolates from Caladenia, a speciose clade of Australian orchids known to associate with Serendipita fungi. DNA sequence data for 7 nuclear and mtDNA loci were used to erect competing species hypotheses by clustering isolates based on: (a) ITS sequence divergence, (b) Bayesian admixture analysis, and (c) mtDNA variation. We implemented two coalescent-based Bayesian methods to determine which species hypothesis best fitted our data. Both methods found strong support for eight species of Serendipita among our isolates, supporting species boundaries reflected in ITS divergence. Patterns of host plant association showed evidence for both generalist and specialist associations within the host genus Caladenia. Our findings demonstrate the utility of Bayesian species delimitation methods and suggest that wider application of these techniques will readily uncover new species in other cryptic fungal lineages.,FUNECO_605_SerendipitaATP6Sequence data: locus ATP6FUNECO_605_SerendipitaC11488Sequence data: locus C11488FUNECO_605_SerendipitaC11804Sequence data: locus C11804FUNECO_605_SerendipitaC16699Sequence data: locus C16699FUNECO_605_SerendipitaC28586Sequence data: locus C28586FUNECO_605_SerendipitaITS1-4Sequence data: locus ITSFUNECO_605_SerendipitaLSUSequence data: locus LSUFUNECO_605_StarbeastXMLStarbeast XML files for four species delimitation hypotheses: T=2, T=3, T=7, and T=8,

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External Organisations
University of Melbourne; Australian National University; Macquarie University; Curtin University; Botanic Gardens and Parks Authority; Western Sydney University
Associated Persons
Kingsley W. Dixon (Creator)Michael R. Whitehead (Creator); Monica Ruibal (Creator); Celeste C. Linde (Creator)

Issued: 2018-01-01

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