Data

Franklin Voyage FR 05/95 Chlorophyll a Data

Australian Ocean Data Network
CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with) Clementson, Lesley (Point of contact)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/2985c975-1ee6-4552-a46d-8ff11534268f&rft.title=Franklin Voyage FR 05/95 Chlorophyll a Data&rft.identifier=Anzlic Identifier: ANZCW0306006432&rft.publisher=Australian Ocean Data Network&rft.description=This record contains data collected from 9 stations on the Franklin cruise FR05/95. The data were collected to determine the chlorophyll a concentration in waters where ther had been a recent mass mortality of pilchards. The data can be used for Ocean Colour sensor validation. Chlorophyll a (HPLC)concentration was the only parameter measured.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the RV Franklin. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis: 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, were determined from standards (Sigma).&rft.creator=Anonymous&rft.date=2004&rft.coverage=westlimit=20; southlimit=-70; eastlimit=127; northlimit=-28&rft.coverage=westlimit=20; southlimit=-70; eastlimit=127; northlimit=-28&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link . Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was collected on voyage [Insert-voyage-name-here] by the Marine National Facility.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Coastal Waters (Australia) | West Australia Coast West, WA&rft_subject=Global / Oceans | Indian Ocean&rft_subject=WfO Biogeochemistry&rft_subject=Research Voyage: FR 05/95&rft_subject=NASDA Ocean Colour Global Research Network System Project l 1994-1999&rft_subject=Ship: Franklin&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link . Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was collected on voyage [Insert-voyage-name-here] by the Marine National Facility.

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This record contains data collected from 9 stations on the Franklin cruise FR05/95. The data were collected to determine the chlorophyll a concentration in waters where ther had been a recent mass mortality of pilchards. The data can be used for Ocean Colour sensor validation. Chlorophyll a (HPLC)concentration was the only parameter measured.

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Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the RV Franklin. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis: 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, were determined from standards (Sigma).

Notes

Credit
Lesley Clementson

Data time period: 1995-06-13 to 1995-06-16

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127,-28 127,-70 20,-70 20,-28 127,-28

73.5,-49

text: westlimit=20; southlimit=-70; eastlimit=127; northlimit=-28

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Coastal Waters (Australia) | West Australia Coast West, WA | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Global / Oceans | Indian Ocean | HPLC (High Performance Liquid Chromatography) | NASDA Ocean Colour Global Research Network System Project l 1994-1999 | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Research Voyage: FR 05/95 | Ship: Franklin | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=627

This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

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Identifiers
  • Local : Anzlic Identifier: ANZCW0306006432
  • Local : Marlin Record Number: 6432
  • global : 2985c975-1ee6-4552-a46d-8ff11534268f