Data

Fractioned eDNA samples collected from Mettam's Pool (WA) using 16S teleost fish, 16S crustaceans, 16S bacteria, and 18S eukaryotes (universal) primers.

Commonwealth Scientific and Industrial Research Organisation
Power, Haylea ; Takahashi, Miwa ; Jarman, Simon ; Berry, Olly
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25919/w751-px86&rft.title=Fractioned eDNA samples collected from Mettam's Pool (WA) using 16S teleost fish, 16S crustaceans, 16S bacteria, and 18S eukaryotes (universal) primers.&rft.identifier=https://doi.org/10.25919/w751-px86&rft.publisher=Commonwealth Scientific and Industrial Research Organisation&rft.description=Sequencing Fastq, LCA, BLAST and ZOTU files for the four metabarcoding assays used in the study, which target teleost fish, crustaceans, bacteria, and eukaryotes (universal). \nLineage: The metabarcoding assay design, amplification and library sequencing were performed as described in Takahashi et al. (2020). Metabarcode sequencing used an Illumina MiSeq platform (V2 chemistry). Fusion PCRs were performed on DNA extracts (with appropriate DNA input determined by qPCR) for each fusion assay with unique multiple identifier (MID) tags.&rft.creator=Power, Haylea &rft.creator=Takahashi, Miwa &rft.creator=Jarman, Simon &rft.creator=Berry, Olly &rft.date=2023&rft.edition=v1&rft.coverage=115.752,-31.8659&rft_rights=Creative Commons Attribution 4.0 International Licence https://creativecommons.org/licenses/by/4.0/&rft_rights=Data is accessible online and may be reused in accordance with licence conditions&rft_rights=All Rights (including copyright) CSIRO 2023.&rft_subject=Environmental DNA (eDNA)&rft_subject=fractionation&rft_subject=teleost fish&rft_subject=crustaceans&rft_subject=bacteria&rft_subject=and eukaryotes (universal)&rft_subject=serial filtering&rft_subject=desired taxa selection&rft_subject=tissue&rft_subject=cells&rft_subject=extracellular DNA&rft_subject=sampling methods.&rft_subject=Environmental assessment and monitoring&rft_subject=Environmental management&rft_subject=ENVIRONMENTAL SCIENCES&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution 4.0 International Licence
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Data is accessible online and may be reused in accordance with licence conditions

All Rights (including copyright) CSIRO 2023.

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Brief description

Sequencing Fastq, LCA, BLAST and ZOTU files for the four metabarcoding assays used in the study, which target teleost fish, crustaceans, bacteria, and eukaryotes (universal).
Lineage: The metabarcoding assay design, amplification and library sequencing were performed as described in Takahashi et al. (2020). Metabarcode sequencing used an Illumina MiSeq platform (V2 chemistry). Fusion PCRs were performed on DNA extracts (with appropriate DNA input determined by qPCR) for each fusion assay with unique multiple identifier (MID) tags.

Available: 2023-11-02

Data time period: 2022-02-21 to 2022-02-21

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115.752,-31.8659

115.752,-31.8659

Subjects
Environmental Sciences | Environmental DNA (eDNA) | Environmental Assessment and Monitoring | Environmental Management | and eukaryotes (universal) | bacteria | cells | crustaceans | desired taxa selection | extracellular DNA | fractionation | sampling methods. | serial filtering | teleost fish | tissue |

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