Data

Evaluation of eDNA as a technique to monitor freshwater fish in southwestern Australia

The University of Western Australia
Beesley, Leah ; Stevens, Emma R. ; Hyde, Josephine ; Gwinn, Daniel ; Morris, Lenore ; Thompson, Suzanne ; Wilson, Paige R. ; Gleeson, Deirdre
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26182/864g-2p23&rft.title=Evaluation of eDNA as a technique to monitor freshwater fish in southwestern Australia&rft.identifier=10.26182/864g-2p23&rft.publisher=The University of Western Australia&rft.description=Our goal was to evaluate eDNA as a monitoring tool for freshwater fish and specifically the use of passive eDNA collection. We had two aims: (1) to determine the effectiveness of active and passive eDNA sampling compared to a conventional technique (fyke netting) to quantify a freshwater fish assemblage in a riverine environment, and (2) to investigate the sampling effort (i.e., replicate number) required to optimize detection of individual species as well as the entire fish assemblage (i.e., species richness). We used a hierarchical multispecies N-mixture model that disentangles the processes of abundance and detection to evaluate the performance of the different sampling methods and to determine the sampling effort required to accurately detect species and describe site-level species richness. This study is one of the few to trial a passive approach to eDNA collection in freshwater. Our findings will assist the advancement and adoption of eDNA monitoring in freshwater ecosystems.&rft.creator=Beesley, Leah &rft.creator=Stevens, Emma R. &rft.creator=Hyde, Josephine &rft.creator=Gwinn, Daniel &rft.creator=Morris, Lenore &rft.creator=Thompson, Suzanne &rft.creator=Wilson, Paige R. &rft.creator=Gleeson, Deirdre &rft.date=2025&rft.relation=http://research-repository.uwa.edu.au/en/publications/edd59338-8226-43c4-89a2-832c0fc6b6b5&rft.coverage=Canning River&rft.coverage=116.021589,-32.118467&rft.coverage=116.021589,-32.118467&rft_rights=&rft_rights=Animal Ethics RA/3/100/1377&rft_subject=Djarlgaroo Beeliar&rft_subject=eDNA&rft_subject=Canning River&rft_subject=fish assemblage&rft_subject=monitoring&rft_subject=freshwater ecology&rft_subject=conservation&rft_subject=management&rft.type=dataset&rft.language=English Access the data
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Animal Ethics RA/3/100/1377

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Our goal was to evaluate eDNA as a monitoring tool for freshwater fish and specifically the use of passive eDNA collection. We had two aims: (1) to determine the effectiveness of active and passive eDNA sampling compared to a conventional technique (fyke netting) to quantify a freshwater fish assemblage in a riverine environment, and (2) to investigate the sampling effort (i.e., replicate number) required to optimize detection of individual species as well as the entire fish assemblage (i.e., species richness). We used a hierarchical multispecies N-mixture model that disentangles the processes of abundance and detection to evaluate the performance of the different sampling methods and to determine the sampling effort required to accurately detect species and describe site-level species richness. This study is one of the few to trial a passive approach to eDNA collection in freshwater. Our findings will assist the advancement and adoption of eDNA monitoring in freshwater ecosystems.

Notes

External Organisations
University of Adelaide; Connecticut Agricultural Experiment Station; Department of Biodiversity, Conservation and Attractions (Western Australia); Biologic Environmental Survey
Associated Persons
Emma R. Stevens (Contributor); Josephine Hyde (Contributor); Suzanne Thompson (Contributor); Paige R. Wilson (Creator)

Created: 2021 to 2021

Issued: 2025-02

Data time period: 2021-04 to 2021-04

This dataset is part of a larger collection

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116.02159,-32.11847

116.021589,-32.118467

text: Canning River

text: 116.021589,-32.118467

Subjects
Canning River | Djarlgaroo Beeliar | conservation | eDNA | fish assemblage | freshwater ecology | management | monitoring |

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