Data

Euphausiid-specific metabarcoding data from eDNA samples and video footage collected on the TEMPO voyage

Australian Antarctic Division
Suter, L. ; SUTER, LEONIE
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/hr9a-jv26&rft.title=Euphausiid-specific metabarcoding data from eDNA samples and video footage collected on the TEMPO voyage&rft.identifier=10.26179/hr9a-jv26&rft.publisher=Australian Antarctic Data Centre&rft.description=This dataset was collected on the TEMPO voyage in 2021 in the Southern Ocean of East Antarctica. The data contains A) genetic data from eDNA samples and B) video footage from a CTD-mounted camera. The eDNA samples were collected by filtering five litres of seawater in the East Antarctic Southern Ocean from the surface and seafloor from CTD samples as well as from the uncontaminated seawater line in and near krill swarms(total n = 110). After DNA extraction, a Euphausiid-specific metabarcoding marker was used to determine the presence of different Southern Ocean krill species in each sample. The CTD-mounted camera collected ~5 min of video footage at the surface and seafloor at each CTD station. Detailed methods are available in the following publication: Suter L et al. (2025) Monitoring Antarctic krill (Euphausia superba) distribution in the Southern Ocean: environmental DNA (eDNA) adds to the toolbox. Frontiers in Marine Science. The data provided here contains- raw paired end sequencing data in fastq format for each sample- R markdown code to process the raw data in order to identify unique sequences, and which sequences are contained in which samples- a tag file that identifies which multiplex-identifier (MID) tags were used in forward and reverse primers for each sample- a zOTU table that identifies which sequences were detected in each sample, and what taxonomy was associated with each sequence.- video footage from each CTD station, both from the surface and the seafloorProgress Code: completed&rft.creator=Suter, L. &rft.creator=SUTER, LEONIE &rft.date=2025&rft.coverage=westlimit=55; southlimit=-68; eastlimit=80; northlimit=-62.83&rft.coverage=westlimit=55; southlimit=-68; eastlimit=80; northlimit=-62.83&rft_rights=These data are publicly available for download from the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_TEMPO_Euphausiid_metabarcoding_data when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=oceans&rft_subject=biota&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > INDIGENOUS/NATIVE SPECIES&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL)&rft_subject=R/V INVESTIGATOR&rft_subject=TEMPO&rft_subject=METABARCODING&rft_subject=CTD > Conductivity, Temperature, Depth&rft_subject=Ships&rft_subject=AMD&rft_subject=CEOS&rft_subject=AMD/AU&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=OCEAN > SOUTHERN OCEAN > EAST ANTARCTIC CONTINENTAL SHELF&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

These data are publicly available for download from the provided URL.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_TEMPO_Euphausiid_metabarcoding_data when using these data.

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Full description

This dataset was collected on the TEMPO voyage in 2021 in the Southern Ocean of East Antarctica. The data contains A) genetic data from eDNA samples and B) video footage from a CTD-mounted camera. The eDNA samples were collected by filtering five litres of seawater in the East Antarctic Southern Ocean from the surface and seafloor from CTD samples as well as from the uncontaminated seawater line in and near krill swarms(total n = 110). After DNA extraction, a Euphausiid-specific metabarcoding marker was used to determine the presence of different Southern Ocean krill species in each sample. The CTD-mounted camera collected ~5 min of video footage at the surface and seafloor at each CTD station.
Detailed methods are available in the following publication:
Suter L et al. (2025) Monitoring Antarctic krill (Euphausia superba) distribution in the Southern Ocean: environmental DNA (eDNA) adds to the toolbox. Frontiers in Marine Science.
The data provided here contains
- raw paired end sequencing data in fastq format for each sample
- R markdown code to process the raw data in order to identify unique sequences, and which sequences are contained in which samples
- a tag file that identifies which multiplex-identifier (MID) tags were used in forward and reverse primers for each sample
- a zOTU table that identifies which sequences were detected in each sample, and what taxonomy was associated with each sequence.
- video footage from each CTD station, both from the surface and the seafloor

Lineage

Progress Code: completed

Data time period: 2021-02-13 to 2021-03-11

This dataset is part of a larger collection

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80,-62.83 80,-68 55,-68 55,-62.83 80,-62.83

67.5,-65.415

text: westlimit=55; southlimit=-68; eastlimit=80; northlimit=-62.83

Subjects
AMD | AMD/AU | CEOS | CTD > Conductivity, Temperature, Depth | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL) | EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > INDIGENOUS/NATIVE SPECIES | GEOGRAPHIC REGION > POLAR | METABARCODING | OCEAN > SOUTHERN OCEAN | OCEAN > SOUTHERN OCEAN > EAST ANTARCTIC CONTINENTAL SHELF | R/V INVESTIGATOR | Ships | TEMPO | biota | oceans |

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Other Information
Download the dataset. (GET DATA > DIRECT DOWNLOAD)

url : https://data.aad.gov.au/eds/6029/download

Public information for AAS project AAS_4556 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/report_project_public.cfm?project_no=4556

Citation reference for this metadata record and dataset. (VIEW RELATED INFORMATION)

url : https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_TEMPO_Euphausiid_metabarcoding_data

Identifiers
ACN 633 798 857