Data

Enzyme analysis of long spined sea urchin (Centrostephanus rodgersii) on the south eastern seaboard of Australia

Australian Ocean Data Network
Johnson, Craig, Prof. ; Miller, Karen, Dr
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/c12bac10-8b33-11dc-8a3c-00188b4c0af8&rft.title=Enzyme analysis of long spined sea urchin (Centrostephanus rodgersii) on the south eastern seaboard of Australia&rft.identifier=https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/c12bac10-8b33-11dc-8a3c-00188b4c0af8&rft.description=Sixty animals were collected from each of Bass Pt, New South Wales (lat 34°35' S, long 150°54' E; August 2000); south side of East Cove, Deal Is, Bass St. (lat 39°28.4' S, long 147°18.4' E; June 2000) and Fortescue Bay, Tasmania (lat 43°8.5' S, long 148°0.0' E; October 2000 and April 2001). To examine the genetic relationship between the three site populations of Centrostephanus rodgersii, allelic diversity and heterozygosity among the three sites was compared using BIOSYS.Maintenance and Update Frequency: notPlannedStatement: Samples of gonad (free of other tissue) were excised from live animals, snap frozen in liquid nitrogen and stored at -80° C. Samples were screened initially for 18 enzyme systems. Five (ADH, AK, GPDH, LDH and 6PGDH) were eliminated from further investigation due to failure to produce detectable banding. Of the remaining 13, seven (AAT, APK, IDH, MDH, MPI, PGM and PGI) provided clearly interpretable and consistently repeatable patterns. Of these, three (APK, IDH, MDH) were monomorphic. The remaining four enzyme systems (AAT, MPI, PGM and PGI) were selected for use in the primary study. MPI, PGM and PGI were all run in the tris-glycene buffer (pH 8.5) and provided one polymorphic locus each. Electrophoretic runs using a tris-glyine buffer were carried out at room temperature over 30 minutes, while those using tris-citrate were run at 4°C for 65 minutes. Protocols for cellulose acetate electrophoresis were after Richardson et al (1986).&rft.creator=Johnson, Craig, Prof. &rft.creator=Miller, Karen, Dr &rft.date=2007&rft.coverage=westlimit=147; southlimit=-39.5; eastlimit=147.5; northlimit=-39&rft.coverage=westlimit=147; southlimit=-39.5; eastlimit=147.5; northlimit=-39&rft.coverage=westlimit=150.5; southlimit=-35; eastlimit=151; northlimit=-34.5&rft.coverage=westlimit=150.5; southlimit=-35; eastlimit=151; northlimit=-34.5&rft.coverage=westlimit=148; southlimit=-43.5; eastlimit=148.5; northlimit=-43&rft.coverage=westlimit=148; southlimit=-43.5; eastlimit=148.5; northlimit=-43&rft.coverage=uplimit=18; downlimit=5&rft.coverage=uplimit=18; downlimit=5&rft_rights=Creative Commons Attribution 2.5 Australia License http://creativecommons.org/licenses/by/2.5/au/&rft_rights=The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).&rft_rights=Consult attached report for a summary of the results. For further information please contact K. Miller.&rft_rights=The data described in this record are the intellectual property of the University of Tasmania through the School of Zoology and the Tasmanian Aquaculture and Fisheries Institute.&rft_subject=biota&rft_subject=25 211001&rft_subject=Centrostephanus rodgersii&rft_subject=isozymes&rft_subject=genetics&rft_subject=ANIMALS/INVERTEBRATES&rft_subject=EARTH SCIENCE&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=REEF HABITAT&rft_subject=BIOSPHERE&rft_subject=AQUATIC ECOSYSTEMS&rft_subject=ECHINODERMS&rft_subject=SEA URCHINS&rft_subject=WATER TEMPERATURE&rft_subject=Temperate Reef&rft_subject=Fish Physiology and Genetics&rft_subject=AGRICULTURAL AND VETERINARY SCIENCES&rft_subject=FISHERIES SCIENCES&rft_subject=Marine and Estuarine Ecology (incl. Marine Ichthyology)&rft_subject=BIOLOGICAL SCIENCES&rft_subject=ECOLOGY&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution 2.5 Australia License
http://creativecommons.org/licenses/by/2.5/au/

The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).

Consult attached report for a summary of the results. For further information please contact K. Miller.

The data described in this record are the intellectual property of the University of Tasmania through the School of Zoology and the Tasmanian Aquaculture and Fisheries Institute.

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Brief description

Sixty animals were collected from each of Bass Pt, New South Wales (lat 34°35' S, long 150°54' E; August 2000); south side of East Cove, Deal Is, Bass St. (lat 39°28.4' S, long 147°18.4' E; June 2000) and Fortescue Bay, Tasmania (lat 43°8.5' S, long 148°0.0' E; October 2000 and April 2001). To examine the genetic relationship between the three site populations of Centrostephanus rodgersii, allelic diversity and heterozygosity among the three sites was compared using BIOSYS.

Lineage

Maintenance and Update Frequency: notPlanned
Statement: Samples of gonad (free of other tissue) were excised from live animals, snap frozen in liquid nitrogen and stored at -80° C. Samples were screened initially for 18 enzyme systems. Five (ADH, AK, GPDH, LDH and 6PGDH) were eliminated from further investigation due to failure to produce detectable banding. Of the remaining 13, seven (AAT, APK, IDH, MDH, MPI, PGM and PGI) provided clearly interpretable and consistently repeatable patterns. Of these, three (APK, IDH, MDH) were monomorphic. The remaining four enzyme systems (AAT, MPI, PGM and PGI) were selected for use in the primary study. MPI, PGM and PGI were all run in the tris-glycene buffer (pH 8.5) and provided one polymorphic locus each. Electrophoretic runs using a tris-glyine buffer were carried out at room temperature over 30 minutes, while those using tris-citrate were run at 4°C for 65 minutes. Protocols for cellulose acetate electrophoresis were after Richardson et al (1986).

Notes

Credit
Funding: FRDC project 2001/044
Purpose
To determine whether newly established populations of the long spined sea urchin (Centrostephanus rodgersii) in Tasmania displayed lower levels of genetic variation relative to populations from mainland Australia, as might be expected if Tasmanian populations arose from a single or small number of founder events with little subsequent gene flow from other populations.

Created: 05 11 2007

Data time period: 06 2000 to 04 2001

This dataset is part of a larger collection

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147.5,-39 147.5,-39.5 147,-39.5 147,-39 147.5,-39

147.25,-39.25

151,-34.5 151,-35 150.5,-35 150.5,-34.5 151,-34.5

150.75,-34.75

148.5,-43 148.5,-43.5 148,-43.5 148,-43 148.5,-43

148.25,-43.25

text: westlimit=147; southlimit=-39.5; eastlimit=147.5; northlimit=-39

text: westlimit=150.5; southlimit=-35; eastlimit=151; northlimit=-34.5

text: westlimit=148; southlimit=-43.5; eastlimit=148.5; northlimit=-43

text: uplimit=18; downlimit=5

Subjects
25 211001 | Agricultural and Veterinary Sciences | ANIMALS/INVERTEBRATES | AQUATIC ECOSYSTEMS | BIOLOGICAL CLASSIFICATION | Biological Sciences | BIOSPHERE | Centrostephanus rodgersii | EARTH SCIENCE | ECHINODERMS | Ecology | Fisheries Sciences | Fish Physiology and Genetics | Marine and Estuarine Ecology (Incl. Marine Ichthyology) | REEF HABITAT | SEA URCHINS | Temperate Reef | WATER TEMPERATURE | biota | genetics | isozymes |

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Other Information
(REPORT - FRDC final report [direct download])

uri : https://data.imas.utas.edu.au/attachments/c12bac10-8b33-11dc-8a3c-00188b4c0af8/FRDC_FINAL_report_Johnson__2001_044____1.pdf

Identifiers
  • global : c12bac10-8b33-11dc-8a3c-00188b4c0af8
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