Data

Environmental DNA data from the RV Investigator South-East Australian Marine Ecosystem Survey (SEA-MES) voyages IN2023_V05 and IN2024_V03 (NESP MaC 4.23)

University of Tasmania, Australia
Deagle, Bruce ; Bodrossy, Levente ; Bessey, Cindy ; Jayasinghe, Sahan ; West, Katrina
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25959/AY48-9W15&rft.title=Environmental DNA data from the RV Investigator South-East Australian Marine Ecosystem Survey (SEA-MES) voyages IN2023_V05 and IN2024_V03 (NESP MaC 4.23)&rft.identifier=10.25959/AY48-9W15&rft.description=This project generated a set of high-quality eDNA-based biodiversity datasets from samples collected at broadly distributed offshore sites across Southeast Australia. The data consists of processed data from eDNA metabarcoding assays (i.e. tables with sampling information, sequence read counts and taxonomy derived from the sequences). Raw sequence data (over 180 million sequences) is available from NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA1263580: Environmental DNA measuring offshore marine biodiversity in southeast Australia (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1263580/).The eDNA biodiversity information focussed on fish but includes sequence data from the full tree of life (from bacteria to vertebrates). Part of the purpose of data collection was to assess key technical aspects of eDNA data collection to refine sampling methods and provide guidance for best practices in future offshore eDNA researchMaintenance and Update Frequency: notPlannedStatement: eDNA samples were collected from the CSIRO research vessel (RV) Investigator as part of the first two SEA-MES voyages: IN2023_V05 (July 2023) and IN2024_V03 (May 2024). Sampling occurred at sites along the edge of the continental shelf (~80 to 500 m) stretching from the east coast of Tasmania to Batemans Bay (NSW). The eDNA samples primarily came from water collected from Niskin bottles on a CTD rosette. An eDNA sampler was also deployed on the deep tow camera system to collect eDNA when this device was towed along transects near the bottom. During the two voyages, water was collected from over 100 CTD deployments, with most samples collected from near the surface and bottom. eDNA metabarcoding was used to obtain sequence information from the samples. This project used several different eDNA markers (also referred to as assays) allowing detection of a broad range of marine species. The total sequencing of eDNA produced over 180 million sequences. Markers included Fish (mtDNA 16S), Metazoan (mtDNA COI Leray) and Eukaryote (V4 18S) and a panel of 12 different markers were generated (targets include bacteria, crustaceans, fish, etc.).&rft.creator=Deagle, Bruce &rft.creator=Bodrossy, Levente &rft.creator=Bessey, Cindy &rft.creator=Jayasinghe, Sahan &rft.creator=West, Katrina &rft.date=2025&rft.coverage=-38.89254198899994,147.59923318200003 -37.81183499399999,148.23130744600007 -37.72573579599998,149.59621055600007 -37.559906309999974,149.76197475100003 -37.505288327999956,149.97650212200006 -37.277661223999985,149.94402256000012 -37.26277983299997,150.0516245440001 -37.12198061699996,149.8782478920001 -37.08324051599995,149.87233939800012 -36.81091852099999,149.94091562500012 -36.265510235999955,150.14813956600005 -35.69112903399997,150.17337033900003 -35.69566224499994,150.29717081900003 -35.46469625199995,150.39286923600002 -35.30187395799993,150.47378954600003 -35.216501662999974,150.53956664700002 -35.167816682999955,150.7560943210001 -35.02213867499996,150.68067515200005 -34.992344981999956,150.76102564400003 -35.07491108999994,150.8327706220001 -34.85195137299998,150.66667197700008 -34.90018518099998,150.76006335700004 -34.31848419299996,150.926796511 -34.03473121899998,151.22542660500005 -34.03570351099995,151.13754781700004 -33.95395971199998,151.16125342500004 -33.96845172199994,151.26599530800002 -33.930496820999956,151.26003341400008 -33.83297822199994,151.28099332500003 -33.869958320999956,151.26766542100003 -33.87469321799995,151.23262332600004 -33.872511712999945,151.17510023500006 -33.870414909999965,151.14163713900007 -33.84062820899993,151.1328304440001 -33.79470681299995,151.21959385800005 -33.796478769999965,151.2485343410001 -33.82321852399996,151.3013208220001 -33.799911117999955,151.304593154 -33.85087872099996,152.13702142300008 -35.009654258999944,151.79789845900007 -36.25648908399996,151.11965253000002 -37.40681181499996,150.8451244150001 -39.555567055999965,150.57059630100002 -43.13064627799997,149.16565830500008 -44.14736541399998,147.3731512060001 -43.514691897999974,146.4673809950001 -43.52326498199993,146.54230112400012 -43.60032880099994,146.78535296200005 -43.60785490399996,146.82019245300012 -43.51596390899994,146.9007949490001 -43.51478780999997,146.90866594700003 -43.54629571099998,146.91727074200003 -43.478112411999966,146.94203324600005 -43.41922130999997,146.9058881620001 -43.340145015999944,146.99603945400008 -43.328347813999976,146.9641925630001 -43.29006052299997,147.10019624000006 -43.278771233999976,147.22673361500006 -43.07323823499996,147.2555524390001 -43.016896035999935,147.2717391440001 -42.99998703999995,147.3278133340001 -42.99998703999995,147.32809703400005 -42.99998703999995,147.3282011340001 -42.99998703999995,147.3285273350001 -43.050479246999934,147.418811408 -42.845972761999974,147.61338789800004 -42.819388080999936,147.86253815100008 -42.66942648899994,147.96306835300004 -42.55638228099997,147.87389368700008 -42.405625388999965,147.98008928800004 -42.10780859499994,148.07753281200007 -42.251990509999985,148.25765215500007 -42.19379081799997,148.35583564400008 -41.81395150799995,148.26361251800006 -41.45723570699994,148.2678170690001 -41.27644521299993,148.35910017800006 -41.22756370699994,148.28283640000006 -41.049876102999974,148.25611033000007 -40.993279109999946,148.34966492 -40.76879308699995,148.0795550040001 -40.744422476999944,147.94952743200008 -40.73901877499997,147.93965383400007 -40.75923197699996,147.9598090280001 -40.815043075999945,147.93324502500002 -40.88657106799997,147.83532503300012 -40.86071905799999,147.70882506200007 -40.820958453999936,147.66954887400004 -41.002473137999985,147.44174539300002 -40.94137302499996,147.28043913300007 -41.06328498099998,146.7071352270001 -40.39704363999995,147.16321794200007 -38.89254198899994,147.59923318200003&rft.coverage=-40.26901348499995,148.08873607200007 -40.210641994999946,148.21041225700003 -40.210038703999935,148.33350513400012 -39.96494119899995,148.291125576 -39.948888290999946,148.17748889900008 -39.72115636999995,147.94474607300003 -39.751184362999936,147.8467713870001 -39.83685496299995,147.84238197600007 -39.873857154999946,147.74749838800005 -40.021672765999945,147.8711253460001 -40.26901348499995,148.08873607200007&rft.coverage=-40.43561411799992,148.48217257400006 -40.39012901399997,148.4319298900001 -40.316491302999964,148.30597912400003 -40.34278549499994,148.20675274000007 -40.418617877999964,147.98726137000006 -40.43561411799992,148.48217257400006&rft.coverage=-42.50931498399995,147.91808498500006 -42.50467548399997,147.92339498500007 -42.50319328399996,147.91894938600012 -42.50931498399995,147.91808498500006&rft_rights=This dataset is hosted by the University of Tasmania, on behalf of CSIRO and NESP Marine and Coastal Hub Project 4.23.&rft_rights=Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0/&rft_rights=Cite data as: Deagle, B., Bodrossy, L., Bessey, C., Jayasinghe, S., & West, K. (2025). Environmental DNA data from the RV Investigator South-East Australian Marine Ecosystem Survey (SEA-MES) voyages IN2023_V05 and IN2024_V03 [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/AY48-9W15&rft_rights=Data was sourced from the NESP Marine and Coastal Hub – the Marine and Coastal Hub is supported through funding from the Australian Government’s National Environmental Science Program (NESP), administered by the Department of Climate Change, Energy, the Environment and Water.&rft_subject=biota&rft_subject=environment&rft_subject=National Environmental Science Program (NESP) Marine and Coastal Hub&rft_subject=Environmental DNA&rft_subject=Southeast Australia Marine Ecosystem Survey&rft_subject=Biomonitoring&rft_subject=Marine Biodiversity Survey&rft_subject=RV Investigator&rft_subject=MARINE ECOSYSTEMS&rft_subject=MARINE ENVIRONMENT MONITORING&rft_subject=Coastal Waters (Australia) | Tasmania Coast East and Southeast, TAS&rft_subject=Regional Seas | Tasman Sea&rft_subject=Marine Planning Regions (Australia) | South-east&rft_subject=Marine Features (Australia) | Bass Strait, TAS/VIC&rft_subject=Environmental Monitoring&rft_subject=ENVIRONMENTAL SCIENCES&rft_subject=ENVIRONMENTAL SCIENCE AND MANAGEMENT&rft_subject=Marine and Estuarine Ecology (incl. Marine Ichthyology)&rft_subject=BIOLOGICAL SCIENCES&rft_subject=ECOLOGY&rft_subject=Population, Ecological and Evolutionary Genetics&rft_subject=GENETICS&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

Open Licence view details
CC-BY

Creative Commons Attribution 4.0 International License
https://creativecommons.org/licenses/by/4.0/

This dataset is hosted by the University of Tasmania, on behalf of CSIRO and NESP Marine and Coastal Hub Project 4.23.

Cite data as: Deagle, B., Bodrossy, L., Bessey, C., Jayasinghe, S., & West, K. (2025). Environmental DNA data from the RV Investigator South-East Australian Marine Ecosystem Survey (SEA-MES) voyages IN2023_V05 and IN2024_V03 [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/AY48-9W15

Data was sourced from the NESP Marine and Coastal Hub – the Marine and Coastal Hub is supported through funding from the Australian Government’s National Environmental Science Program (NESP), administered by the Department of Climate Change, Energy, the Environment and Water.

Access:

Open

Full description

This project generated a set of high-quality eDNA-based biodiversity datasets from samples collected at broadly distributed offshore sites across Southeast Australia. The data consists of processed data from eDNA metabarcoding assays (i.e. tables with sampling information, sequence read counts and taxonomy derived from the sequences). Raw sequence data (over 180 million sequences) is available from NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA1263580: Environmental DNA measuring offshore marine biodiversity in southeast Australia (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1263580/).

The eDNA biodiversity information focussed on fish but includes sequence data from the full tree of life (from bacteria to vertebrates). Part of the purpose of data collection was to assess key technical aspects of eDNA data collection to refine sampling methods and provide guidance for best practices in future offshore eDNA research

Lineage

Maintenance and Update Frequency: notPlanned
Statement: eDNA samples were collected from the CSIRO research vessel (RV) Investigator as part of the first two SEA-MES voyages: IN2023_V05 (July 2023) and IN2024_V03 (May 2024). Sampling occurred at sites along the edge of the continental shelf (~80 to 500 m) stretching from the east coast of Tasmania to Batemans Bay (NSW). The eDNA samples primarily came from water collected from Niskin bottles on a CTD rosette. An eDNA sampler was also deployed on the deep tow camera system to collect eDNA when this device was towed along transects near the bottom. During the two voyages, water was collected from over 100 CTD deployments, with most samples collected from near the surface and bottom. eDNA metabarcoding was used to obtain sequence information from the samples. This project used several different eDNA markers (also referred to as assays) allowing detection of a broad range of marine species. The total sequencing of eDNA produced over 180 million sequences. Markers included Fish (mtDNA 16S), Metazoan (mtDNA COI Leray) and Eukaryote (V4 18S) and a panel of 12 different markers were generated (targets include bacteria, crustaceans, fish, etc.).

Notes

Credit
The data collections described in this record are funded by the Australian Government Department of Climate Change, Energy, the Environment and Water (DCCEEW) through the NESP Marine and Coastal Hub. In addition to NESP (DCCEEW) funding, this project is matched by an equivalent amount of in-kind support and co-investment from project partners and collaborators.
Credit
We also acknowledge the use of the CSIRO Marine National Facility (https://ror.org/01mae9353) and grant of sea time on RV Investigator in undertaking this research. We thank the entire South-East Australian Marine Ecosystem Survey (SEA-MES) team, in particular Rich Little (CSIRO), Chief Scientist on the SEA-MES voyages, whose support was key to incorporating eDNA into the broader research program.

Issued: 20 10 2025

Data time period: 2023-07-01 to 2024-06-01

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related Data

Related People

Related Program

Related Grants and Projects

-44.14737,86 -33.79471,86

-38.9710361135,90

-40.26901,86 -39.72116,86

-39.9950849275,90

-40.43561,86 -40.31649,86

-40.3760527105,90

-42.50931,86 -42.50319,86

-42.506254134,90

Subjects
Biological Sciences | Biomonitoring | Coastal Waters (Australia) | Tasmania Coast East and Southeast, TAS | Ecology | Environmental Science and Management | Environmental Sciences | Environmental DNA | Environmental Monitoring | Genetics | MARINE ECOSYSTEMS | MARINE ENVIRONMENT MONITORING | Marine Biodiversity Survey | Marine Features (Australia) | Bass Strait, TAS/VIC | Marine Planning Regions (Australia) | South-east | Marine and Estuarine Ecology (Incl. Marine Ichthyology) | National Environmental Science Program (NESP) Marine and Coastal Hub | Population, Ecological and Evolutionary Genetics | RV Investigator | Regional Seas | Tasman Sea | Southeast Australia Marine Ecosystem Survey | biota | environment |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Other Information
(DATA ACCESS - browse and download available files)

url : https://data.imas.utas.edu.au/attachments/98fb2a49-4082-4648-a618-19270f554e88

Deagle BE, Jayasinghe SA, Bessey C, Bodrossy L, West, KM (2025) Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us? Report to the National Environmental Science Program. CSIRO, Hobart, Australia. (Final Project Report)

url : https://www.nespmarinecoastal.edu.au/publication/environmental-dna-for-measuring-offshore-marine-biodiversity-what-can-dna-in-water-collected-from-the-rv-investigator-tell-us/

global : c58e91fb-a580-46d9-a7b3-659396c76d3e

local : 05bgxxb69

local : 0000-0001-7651-3687

local : 05bgxxb69

local : 0000-0001-6940-452X

local : 05bgxxb69

local : 0000-0002-4081-0214

local : 05bgxxb69

local : 0000-0002-0073-5301

local : 05bgxxb69

local : 0000-0002-9026-5058

local : 05bgxxb69

local : 0000-0001-7651-3687

NESP MaC Project 4.23 - Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us?, 2024-2025 (CSIRO)

doi : 10.71676/d0dfa7a2

CSIRO Data Access Portal copy of dataset

doi : 10.25919/ngqx-3557

Identifiers
ACN 633 798 857