Data

Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems

Australian Antarctic Data Centre
CLARKE, LAURENCE ; SUTER, LEONIE ; POLANOWSKI, ANDREA ; DEAGLE, BRUCE ; STARK, JONNY ; JOHNSTONE, GLENN JAMES
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/caaz-b854&rft.title=Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems&rft.identifier=10.26179/caaz-b854&rft.publisher=Australian Antarctic Data Centre&rft.description=Our aim was to compare water and sediment as sources of environmental DNA (eDNA) to better characterise Antarctic benthic communities and further develop practical approaches for DNA-based biodiversity assessment in remote environments. We used a cytochrome c oxidase subunit I (COI) metabarcoding approach to characterise metazoan communities in 26 nearshore sites across 12 locations (including Ellis Fjord, Warriner Channel, Hawker Channel, Abatus Bay, Powell Point, Shirokaya Bay, and Weddell Arm) in the Vestfold Hills (East Antarctica) based on DNA extracted from either sediment cores or filtered seawater. We detected a total of 99 metazoan species from 12 phyla (including nematodes, cnidaria, echinoderms, chordates, arthropods, annelids, rotifers and molluscs) across 26 sites, with similar numbers of species detected in sediment and water eDNA samples. Please cite: Clarke LJ et al. (2021). Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems. PeerJ, DOI: 10.7717/peerj.12458 This work was completed as part of the Davis Aerodrome Project (DAP).&rft.creator=CLARKE, LAURENCE &rft.creator=SUTER, LEONIE &rft.creator=POLANOWSKI, ANDREA &rft.creator=DEAGLE, BRUCE &rft.creator=STARK, JONNY &rft.creator=JOHNSTONE, GLENN JAMES &rft.date=2021&rft.coverage=northlimit=-68.42426; southlimit=-68.63487; westlimit=77.88451; eastLimit=78.39757; projection=WGS84&rft.coverage=northlimit=-68.42426; southlimit=-68.63487; westlimit=77.88451; eastLimit=78.39757; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_5097_DAP_nearshore_benthic_DNA_metabarcoding when using these data.&rft_subject=biota&rft_subject=oceans&rft_subject=MOLLUSKS&rft_subject=EARTH SCIENCE&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=ANIMALS/INVERTEBRATES&rft_subject=ROTIFERS&rft_subject=SEGMENTED WORMS (ANNELIDS)&rft_subject=ARTHROPODS&rft_subject=ECHINODERMS&rft_subject=CNIDARIANS&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC&rft_subject=ENVIRONMENTAL DNA&rft_subject=DNA METABARCODING&rft_subject=CYTOCHROME C OXIDASE SUBUNIT I (COI)&rft_subject=METAZOAN&rft_subject=NEMATODES&rft_subject=CNIDARIA&rft_subject=CHORDATES&rft_subject=ANNELIDS&rft_subject=MOLLUSCS&rft_subject=ILLUMINA MISEQ&rft_subject=ADS > Automated DNA Sequencer&rft_subject=LABORATORY&rft_subject=GEOGRAPHIC REGION > POLAR&rft_subject=CONTINENT > ANTARCTICA > VESTFOLD HILLS&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_5097_DAP_nearshore_benthic_DNA_metabarcoding when using these data.

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Brief description

Our aim was to compare water and sediment as sources of environmental DNA (eDNA) to better characterise Antarctic benthic communities and further develop practical approaches for DNA-based biodiversity assessment in remote environments. We used a cytochrome c oxidase subunit I (COI) metabarcoding approach to characterise metazoan communities in 26 nearshore sites across 12 locations (including Ellis Fjord, Warriner Channel, Hawker Channel, Abatus Bay, Powell Point, Shirokaya Bay, and Weddell Arm) in the Vestfold Hills (East Antarctica) based on DNA extracted from either sediment cores or filtered seawater. We detected a total of 99 metazoan species from 12 phyla (including nematodes, cnidaria, echinoderms, chordates, arthropods, annelids, rotifers and molluscs) across 26 sites, with similar numbers of species detected in sediment and water eDNA samples.

Please cite: Clarke LJ et al. (2021). Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems. PeerJ, DOI: 10.7717/peerj.12458

This work was completed as part of the Davis Aerodrome Project (DAP).

Issued: 2021-11-26

Data time period: 2019-11-13 to 2019-12-14

This dataset is part of a larger collection

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78.39757,-68.42426 78.39757,-68.63487 77.88451,-68.63487 77.88451,-68.42426 78.39757,-68.42426

78.14104,-68.529565

text: northlimit=-68.42426; southlimit=-68.63487; westlimit=77.88451; eastLimit=78.39757; projection=WGS84

Subjects
ADS > Automated DNA Sequencer | ANIMALS/INVERTEBRATES | ANNELIDS | ARTHROPODS | BIOLOGICAL CLASSIFICATION | CHORDATES | CNIDARIA | CNIDARIANS | CONTINENT > ANTARCTICA > VESTFOLD HILLS | CYTOCHROME C OXIDASE SUBUNIT I (COI) | DNA METABARCODING | EARTH SCIENCE | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC | ECHINODERMS | ENVIRONMENTAL DNA | GEOGRAPHIC REGION > POLAR | ILLUMINA MISEQ | LABORATORY | METAZOAN | MOLLUSCS | MOLLUSKS | NEMATODES | ROTIFERS | SEGMENTED WORMS (ANNELIDS) | biota | oceans |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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