Data

Environmental DNA (eDNA) survey of Bathurst Harbour (Tasmania) for the endangered Maugean skate (Zearaja maugeana) (NESP MaC 1.33)

University of Tasmania, Australia
Moreno, David ; Semmens, Jayson
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
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Even though it was first discovered in Bathurst Harbour, most of what is known about the species comes from the Macquarie Harbour population. In fact, only four individuals have ever been reported in Bathurst Harbour, with the last known sighting occurring in 1992. This study aimed to use eDNA to determine the presence of the Maugean skate in Bathurst Harbour on the southwest coast of Tasmania. Water samples were collected within 1 m from the sea floor in Bathurst Harbour in November 2021 and February 2022, and in Macquarie Harbour (control samples) in December 2021. Samples were filtered using a self-preserving eDNA sampling system. Following each survey DNA from the samples was extracted and analysed through qPCR amplification. Mitochondrial primer pairs from two gene regions were used to detect the presence of Maugean skate DNA in the samples. Where possible, positive detections were sequenced, and their identity verified.Maintenance and Update Frequency: none-plannedStatement: There were 20 sample sites in Bathurst Harbour, five sites in the Bathurst Channel and one site in Melaleuca Lagoon. There were 2 control sites in Macquarie Harbour at locations known to host Maugean skate. All Bathurst Harbour sites were sampled in both November 2021 and February 2022, and Macquarie Harbour control sites were sampled in December 2021. Two replicate water samples were collected from 1 m above the seafloor using a camera bottle. Each sample was thoroughly shaken and filtration through (5 µm filters and/or 0.45 µm filters) occurred immediately after collection to ensure no degradation of eDNA. 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Marine Ichthyology)&rft_subject=BIOLOGICAL SCIENCES&rft_subject=ECOLOGY&rft_subject=Wildlife and Habitat Management&rft_subject=ENVIRONMENTAL SCIENCES&rft_subject=ENVIRONMENTAL SCIENCE AND MANAGEMENT&rft.type=dataset&rft.language=English Access the data
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This dataset is hosted by the Institute for Marine and Antarctic Studies (IMAS), University of Tasmania, on behalf of NESP Marine and Coastal Hub Project 1.33.

Data was sourced from the NESP Marine and Coastal Hub – the Marine and Coastal Hub is supported through funding from the Australian Government’s National Environmental Science Program (NESP), administered by the Department of Climate Change, Energy, the Environment and Water (DCCEEW).

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Brief description

The Maugean Skate Zearaja maugeana is a micro-endemic species known from only two isolated estuaries, Bathurst and Macquarie Harbours in southwestern/western Tasmania. This constitutes one of the most limited distributions of any known extant elasmobranch. As a result, the species is listed as ‘Endangered’ under the Threatened Species Protection Act (Tasmania) and the Environmental Protection and Biodiversity Conservation Act (Commonwealth).

Even though it was first discovered in Bathurst Harbour, most of what is known about the species comes from the Macquarie Harbour population. In fact, only four individuals have ever been reported in Bathurst Harbour, with the last known sighting occurring in 1992.

This study aimed to use eDNA to determine the presence of the Maugean skate in Bathurst Harbour on the southwest coast of Tasmania.

Water samples were collected within 1 m from the sea floor in Bathurst Harbour in November 2021 and February 2022, and in Macquarie Harbour (control samples) in December 2021. Samples were filtered using a self-preserving eDNA sampling system. Following each survey DNA from the samples was extracted and analysed through qPCR amplification. Mitochondrial primer pairs from two gene regions were used to detect the presence of Maugean skate DNA in the samples. Where possible, positive detections were sequenced, and their identity verified.

Lineage

Maintenance and Update Frequency: none-planned
Statement: There were 20 sample sites in Bathurst Harbour, five sites in the Bathurst Channel and one site in Melaleuca Lagoon. There were 2 control sites in Macquarie Harbour at locations known to host Maugean skate. All Bathurst Harbour sites were sampled in both November 2021 and February 2022, and Macquarie Harbour control sites were sampled in December 2021.

Two replicate water samples were collected from 1 m above the seafloor using a camera bottle. Each sample was thoroughly shaken and filtration through (5 µm filters and/or 0.45 µm filters) occurred immediately after collection to ensure no degradation of eDNA.

To provide a positive control, water was sampled from a 750L tank immediately after it had hosted two Maugean skate (captured in Macquarie Harbour in December 2021).

Data time period: 2021-11-03 to 2022-02-09

This dataset is part of a larger collection

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Subjects
Biological Sciences | Bathurst Harbour | EARTH SCIENCE | AGRICULTURE | ANIMAL SCIENCE | ANIMAL ECOLOGY AND BEHAVIOR | EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | ANIMALS/VERTEBRATES | FISH | SHARKS/RAYS/CHIMAERAS | EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | SPECIES/POPULATION INTERACTIONS | ENDANGERED SPECIES | EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | BENTHIC | Ecology | Environmental Science and Management | Environmental Sciences | Macquarie Harbour | Marine and Estuarine Ecology (Incl. Marine Ichthyology) | Maugean skate | National Environmental Science Program (NESP) Marine and Coastal Hub | Wildlife and Habitat Management | Zearaja maugeana | biota | eDNA |

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Other Information
(DATA ACCESS - Maugean skate eDNA qPCR summary results [direct download])

uri : https://data.imas.utas.edu.au/attachments/0fe29a80-b529-4230-8878-fe088d799ee1/NESP_Maugean_skate_eDNA_qPCR_summary_Final.xlsx

(Final report [PDF])

uri : https://www.nespmarinecoastal.edu.au/project-1-33-final-report-2/

(Project website)

uri : https://www.nespmarinecoastal.edu.au/project-1-33-2/

global : 7fcf99b3-b95c-489a-bc4c-f4f1b75af9f2

Identifiers
  • global : 0fe29a80-b529-4230-8878-fe088d799ee1
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