Effects of Short-Term Exercise on Plasma Metabolic and Lipidomic Profiles of Individuals with Type 2 Diabetes

Full description

"NmrControlsDiabetesFasted" is a quantified 1H NMR dataset of 198 healthy controls, 208 people with type 2 diabetes mellitus (T2DM) who acted as disease controls, and 13 people with T2DM who participated in a twelve-day exercise intervention. Blood samples were collected cross-sectionally for healthy controls and T2DM-controls, whereas data from two time points (i.e., pre- and post-intervention) are provided for T2DM participants of the exercise intervention. All samples in this dataset were collected in a fasted state. "NmrLipidsDiabetes" is a dataset of 13 people with T2DM who participated in a twelve-day exercise intervention. As part of the study, participants completed an oral glucose tolerance test (OGTT) before (OGTT1) and after (OGTT2) the twelve-day intervention. Fasted blood samples were collected prior to consuming a drink containing 75g of glucose and at 30-minute intervals until reaching the two-hour mark (i.e., at 30, 60, 90, and 120 minutes post-consumption). Additionally, blood samples were collected before (Pre) and immediately after (Post) exercise training on the first (Ex1) and last day (Ex12) of the intervention. Samples around the exercise sessions were only collected for nine participants. The OGTT and exercise samples were analysed using 1H NMR and lipidomics LC-MS methods. NMR data were collected on Bruker 600 MHz Avance III HD spectrometers equipped with a BBI probe and with integral Bruker SampleJet robots, and data were automatically processed using Bruker Topspin™ 3.6.2 and ICON NMR to enable phasing, baseline correction, and calibration to TSP (δ = 0) (Dona et al., 2014; Lodge et al., 2021; Lodge et al., 2024). Liquid chromatography was performed by a SCIEX ExionLC (SCIEX, Concord, CA) where separation was conducted using Waters Acquity BEH C18 1.7 μm, 2.1 × 100 mm column (Waters Corp., MA, USA) at 60°C (Ryan et al., 2023). Mass detection was conducted by a SCIEX QTRAP 6500+ (SCIEX, Concord, CA) with electrospray ionisation and polarity switching (Ryan et al., 2023). Demographics of the study population and a detailed description of sample preparation and analysis are provided in the journal article associated with this dataset. A reference to this paper will be provided upon publication.

Notes

External Organisations
Australian National Phenome Centre; Murdoch University; Imperial College London

Associated Persons
Maartje Cox (Creator); Aaron Raman (Creator); Timothy Fairchild (Creator); Julien Wist (Creator); Jeremiah Peiffer (Creator); Nathan Lawler (Creator)