Data

eDNA metabarcoding data for the publication 'Passive environment DNA (eDNA) sampling is effective for monitoring vessel biofouling in the Southern Ocean'

Australian Antarctic Division

Perry, F., Shaw, J., Cassey, P., Coutts, A., Lewis, P., MacDonald, A., Polanowski, A., Stark, J and Suter, L. ; SUTER, LEONIE ; PERRY, FRANCES ; SHAW, JUSTINE ; CASSEY, PHILLIP ; COUTTS, ASHLEY ; LEWIS, PATRICK ; MACDONALD, ANNA ; POLANOWSKI, ANDREA ; STARK, JONNY

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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/4m4h-kz60&rft.title=eDNA metabarcoding data for the publication 'Passive environment DNA (eDNA) sampling is effective for monitoring vessel biofouling in the Southern Ocean'&rft.identifier=10.26179/4m4h-kz60&rft.publisher=Australian Antarctic Data Centre&rft.description=This data accompanies the paper 'Passive environment DNA (eDNA) sampling is effective for monitoring vessel biofouling in the Southern Ocean'. Samples were collected from the MPOV Aiviq in the Port of Hobart (-42.87, 147.34) and Macquarie Island (-54.500, 158.950) between September 2022 and October 2022. Passive absorption was used to collect eDNA, with perforated plastic balls with cellulose sponges inserted inside the balls were used as the eDNA absorption material. Samples were collected at four time points, prior to in-water cleaning (Hobart), during in-water cleaning (Hobart), post in-water cleaning (Hobart) and at Macquarie Island. All eDNA samples were amplified with two universal metabarcoding markers targeting the 18S rRNA gene V1-V3 region and part of the mitochondrial cytochrome c oxidase subunit I (COI) gene to gain a wide overview of taxa present. For species-level identification of arthropod and mollusc taxa, samples were also amplified with two group-specific metabarcoding primer sets, each targeting a region of the 16S rRNA gene For each sampling period, we have included the raw, unmerged FASTQ data for each marker, the zOUT fasta files and the processed zOTU table with taxonomy. The R-files for the processing pipeline of FASTQ files and data analysis pipeline are also included to complete all the analysis detailed in the manuscript.Progress Code: completed&rft.creator=Perry, F., Shaw, J., Cassey, P., Coutts, A., Lewis, P., MacDonald, A., Polanowski, A., Stark, J and Suter, L. &rft.creator=SUTER, LEONIE &rft.creator=PERRY, FRANCES &rft.creator=SHAW, JUSTINE &rft.creator=CASSEY, PHILLIP &rft.creator=COUTTS, ASHLEY &rft.creator=LEWIS, PATRICK &rft.creator=MACDONALD, ANNA &rft.creator=POLANOWSKI, ANDREA &rft.creator=STARK, JONNY &rft.date=2025&rft.coverage=westlimit=146.95312; southlimit=-55.07837; eastlimit=159.96094; northlimit=-42.22852&rft.coverage=westlimit=146.95312; southlimit=-55.07837; eastlimit=159.96094; northlimit=-42.22852&rft.coverage=westlimit=146.77734; southlimit=-55.72711; eastlimit=160.48828; northlimit=-42.35854&rft.coverage=westlimit=146.77734; southlimit=-55.72711; eastlimit=160.48828; northlimit=-42.35854&rft_rights=These data are not yet publicly available for download.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_Aiviq when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=transportation&rft_subject=environment&rft_subject=oceans&rft_subject=EARTH SCIENCE > HUMAN DIMENSIONS > INFRASTRUCTURE > TRANSPORTATION&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > EXOTIC SPECIES&rft_subject=EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > WATER RESOURCES&rft_subject=EARTH SCIENCE > ENVIRONMENTAL ADVISORIES > MARINE ADVISORIES > MARINE BIOLOGY&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC > OCEANIC ZONE&rft_subject=EARTH SCIENCE > OCEANS > MARINE ENVIRONMENT MONITORING > MARINE SURFACE ELEMENTS > MARINE VESSELS&rft_subject=MPOV AIVIQ&rft_subject=PCR&rft_subject=ENVIRONMENTAL DNA&rft_subject=BIOFOULING&rft_subject=BIOSECURITY&rft_subject=EDNA&rft_subject=ADS > Automated DNA Sequencer&rft_subject=Ships&rft_subject=AMD&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND&rft_subject=CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > HOBART&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data

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Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

These data are not yet publicly available for download.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_Aiviq when using these data.

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Full description

This data accompanies the paper 'Passive environment DNA (eDNA) sampling is effective for monitoring vessel biofouling in the Southern Ocean'.

Samples were collected from the MPOV Aiviq in the Port of Hobart (-42.87, 147.34) and Macquarie Island (-54.500, 158.950) between September 2022 and October 2022. Passive absorption was used to collect eDNA, with perforated plastic balls with cellulose sponges inserted inside the balls were used as the eDNA absorption material.

Samples were collected at four time points, prior to in-water cleaning (Hobart), during in-water cleaning (Hobart), post in-water cleaning (Hobart) and at Macquarie Island. All eDNA samples were amplified with two universal metabarcoding markers targeting the 18S rRNA gene V1-V3 region and part of the mitochondrial cytochrome c oxidase subunit I (COI) gene to gain a wide overview of taxa present. For species-level identification of arthropod and mollusc taxa, samples were also amplified with two group-specific metabarcoding primer sets, each targeting a region of the 16S rRNA gene

For each sampling period, we have included the raw, unmerged FASTQ data for each marker, the zOUT fasta files and the processed zOTU table with taxonomy. The R-files for the processing pipeline of FASTQ files and data analysis pipeline are also included to complete all the analysis detailed in the manuscript.

Lineage

Progress Code: completed

Data time period: 2022-09-19 to 2022-10-26

This dataset is part of a larger collection

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text: westlimit=146.95312; southlimit=-55.07837; eastlimit=159.96094; northlimit=-42.22852

text: westlimit=146.77734; southlimit=-55.72711; eastlimit=160.48828; northlimit=-42.35854

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Other Information

Download the dataset. (GET DATA > DIRECT DOWNLOAD)

url : https://data.aad.gov.au/eds/6147/download External Link

Public information for AAS project AAS_4556 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/report_project_public.cfm?project_no=AAS_4556 External Link

Citation reference for this metadata record and dataset. (VIEW RELATED INFORMATION)

url : https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_Aiviq External Link

Identifiers

DOI : 10.26179/4M4H-KZ60
global : AAS_4556_Aiviq