Data

Ecophysiology of three habitat-forming seaweeds across a depth and latitudinal gradient in southeastern Australia

University of Tasmania, Australia
Flukes, Emma ; Johnson, Craig ; Wright, Jeffrey
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.4226/77/57216ba85940c&rft.title=Ecophysiology of three habitat-forming seaweeds across a depth and latitudinal gradient in southeastern Australia&rft.identifier=10.4226/77/57216ba85940c&rft.description=Seasonal patterns in the in situ ecophysiology of the common habitat-forming seaweeds Ecklonia radiata, Phyllospora comosa, and Macrocystis pyrifera were investigated at different latitudes and depths in southeastern Australia. We used multiple performance indicators (photosynthetic characteristics, pigment content, chemical composition, stable isotopes, nucleic acids) to assess the ecophysiology of seaweeds near the northern and southern margins of their range, along a depth gradient (E. radiata only), over a two year period (September 2010 – August 2012).Maintenance and Update Frequency: notPlannedStatement: Seaweeds were sampled quarterly in Tasmania, and every 6 months in NSW for 2 years (total of 8 sampling occasions for Tas and 4 for NSW). Ecklonia radiata was sampled at a depth of ~10 m and ~30 m, Phyllospora radiata was sampled at ~6 m, and Macrocystis pyrifera was sampled from the subsurface canopy (~0.5-1 m). Sampling consisted of PAM fluorometry measurements and collection of tissue samples for the following measurements: • Rapid Light Curves (light- and dark-adapted) using PAM fluorometry in situ - parameters are derived from fitted RLCs using the double exponential decay model of Platt et al (1980) • Pigment concentrations (chlorophyll a, c, and fucoxanthin) using HPLC and spectrophotometry) • Tissue chemistry (%C, %N, C:N, stable isotopes δ13C and δ15N) using a thermo gas chromatograph and isotope radio mass spectrometer • Nucleic acid characteristics (concentration of RNA and DNA, RNA:DNA) using a novel extraction technique developed specifically for brown macroalgaeStatement: A blue LED diving-PAM (Waltz) was used for all fluorometry. A Thermo Gas Chromatograph coupled to a Finnigan Mat Delta S isotope radio mass spectrometer operating in continuous flow mode was used for all tissue chemistry measurements. Fucoxanthin was measured using a Waters Acquity H-series UPLC™ coupled to a Waters Acquity Photodiode Array Detector with an Ethylene Bridged Hybrid (BEH) C-18 column (2.1 x 100 mm x 1.7 micron particles). Chlorophyll a and c were measured using a Dynamica HALO RB-10 Spectrophotometer.&rft.creator=Flukes, Emma &rft.creator=Johnson, Craig &rft.creator=Wright, Jeffrey &rft.date=2020&rft.coverage=westlimit=147.90; southlimit=-43.25; eastlimit=148.05; northlimit=-43.10&rft.coverage=westlimit=147.90; southlimit=-43.25; eastlimit=148.05; northlimit=-43.10&rft.coverage=westlimit=152.15977; southlimit=-32.75; eastlimit=152.35; northlimit=-32.55&rft.coverage=westlimit=152.15977; southlimit=-32.75; eastlimit=152.35; northlimit=-32.55&rft.coverage=uplimit=31; downlimit=4&rft.coverage=uplimit=31; downlimit=4&rft_rights=The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies.&rft_rights= http://creativecommons.org/licenses/by/4.0/&rft_rights=http://i.creativecommons.org/l/by/4.0/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution 4.0 International License&rft_rights=http://creativecommons.org/international/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Cite data as: Flukes, E., Johnson, C., & Wright, J. (2015). Ecophysiology of three habitat-forming seaweeds across a depth and latitudinal gradient in southeastern Australia [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.4226/77/57216BA85940C&rft_rights=Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0&rft_subject=biota&rft_subject=kelp&rft_subject=PAM fluorometry&rft_subject=ecophysiology&rft_subject=RNA:DNA&rft_subject=nucleic acid&rft_subject=fucoxanthin&rft_subject=mass spectrometry&rft_subject=HPLC&rft_subject=Ecklonia radiata&rft_subject=Macrocystis pyrifera&rft_subject=Phyllospora comosa&rft_subject=EARTH SCIENCE | BIOSPHERE | VEGETATION | CHLOROPHYLL&rft_subject=EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS)&rft_subject=EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) | BROWN ALGAE&rft_subject=EARTH SCIENCE | BIOSPHERE | VEGETATION | PIGMENTS&rft_subject=EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS&rft_subject=EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | REEF&rft_subject=EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | BENTHIC&rft_subject=EARTH SCIENCE | BIOSPHERE | VEGETATION | NUTRIENTS&rft_subject=Temperate Reef&rft_subject=Marine and Estuarine Ecology (incl. Marine Ichthyology)&rft_subject=BIOLOGICAL SCIENCES&rft_subject=ECOLOGY&rft_subject=Phycology (incl. Marine Grasses)&rft_subject=PLANT BIOLOGY&rft_subject=Ecological Physiology&rft_subject=Ecological Impacts of Climate Change&rft_subject=ENVIRONMENTAL SCIENCES&rft_subject=ECOLOGICAL APPLICATIONS&rft_subject=diver&rft.type=dataset&rft.language=English Access the data
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Cite data as: Flukes, E., Johnson, C., & Wright, J. (2015). Ecophysiology of three habitat-forming seaweeds across a depth and latitudinal gradient in southeastern Australia [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.4226/77/57216BA85940C

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Brief description

Seasonal patterns in the in situ ecophysiology of the common habitat-forming seaweeds Ecklonia radiata, Phyllospora comosa, and Macrocystis pyrifera were investigated at different latitudes and depths in southeastern Australia. We used multiple performance indicators (photosynthetic characteristics, pigment content, chemical composition, stable isotopes, nucleic acids) to assess the ecophysiology of seaweeds near the northern and southern margins of their range, along a depth gradient (E. radiata only), over a two year period (September 2010 – August 2012).

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Maintenance and Update Frequency: notPlanned
Statement: Seaweeds were sampled quarterly in Tasmania, and every 6 months in NSW for 2 years (total of 8 sampling occasions for Tas and 4 for NSW). Ecklonia radiata was sampled at a depth of ~10 m and ~30 m, Phyllospora radiata was sampled at ~6 m, and Macrocystis pyrifera was sampled from the subsurface canopy (~0.5-1 m). Sampling consisted of PAM fluorometry measurements and collection of tissue samples for the following measurements: • Rapid Light Curves (light- and dark-adapted) using PAM fluorometry in situ - parameters are derived from fitted RLCs using the double exponential decay model of Platt et al (1980) • Pigment concentrations (chlorophyll a, c, and fucoxanthin) using HPLC and spectrophotometry) • Tissue chemistry (%C, %N, C:N, stable isotopes δ13C and δ15N) using a thermo gas chromatograph and isotope radio mass spectrometer • Nucleic acid characteristics (concentration of RNA and DNA, RNA:DNA) using a novel extraction technique developed specifically for brown macroalgae
Statement: A blue LED diving-PAM (Waltz) was used for all fluorometry. A Thermo Gas Chromatograph coupled to a Finnigan Mat Delta S isotope radio mass spectrometer operating in continuous flow mode was used for all tissue chemistry measurements. Fucoxanthin was measured using a Waters Acquity H-series UPLC™ coupled to a Waters Acquity Photodiode Array Detector with an Ethylene Bridged Hybrid (BEH) C-18 column (2.1 x 100 mm x 1.7 micron particles). Chlorophyll a and c were measured using a Dynamica HALO RB-10 Spectrophotometer.

Notes

Credit
Australian Research Council (ARC) grant DP1096573
Purpose
To determine similarities and differences in the ecophysiology of temperate habitat-forming seaweeds across a depth and latitudinal gradient.

Data time period: 2010-09-27 to 2012-08-07

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148.05,-43.1 148.05,-43.25 147.9,-43.25 147.9,-43.1 148.05,-43.1

147.975,-43.175

152.35,-32.55 152.35,-32.75 152.15977,-32.75 152.15977,-32.55 152.35,-32.55

152.254885,-32.65

text: westlimit=147.90; southlimit=-43.25; eastlimit=148.05; northlimit=-43.10

text: westlimit=152.15977; southlimit=-32.75; eastlimit=152.35; northlimit=-32.55

text: uplimit=31; downlimit=4

Subjects
Biological Sciences | EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) | EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) | BROWN ALGAE | EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS | EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | BENTHIC | EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | REEF | EARTH SCIENCE | BIOSPHERE | VEGETATION | CHLOROPHYLL | EARTH SCIENCE | BIOSPHERE | VEGETATION | NUTRIENTS | EARTH SCIENCE | BIOSPHERE | VEGETATION | PIGMENTS | Ecological Applications | Ecology | Environmental Sciences | Ecklonia radiata | Ecological Impacts of Climate Change | Ecological Physiology | HPLC | Macrocystis pyrifera | Marine and Estuarine Ecology (Incl. Marine Ichthyology) | PAM fluorometry | Plant Biology | Phycology (Incl. Marine Grasses) | Phyllospora comosa | RNA:DNA | Temperate Reef | biota | diver | ecophysiology | fucoxanthin | kelp | mass spectrometry | nucleic acid |

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