Discovery Projects - Grant ID: DP150100956 [ 2015 - 2017 ]

Research Grant

Researchers Prof Antoine van Oijen; Prof Nicholas Dixon

Brief description The replisome is the machine that makes DNA in cells that are about to divide. Replisomes face many mechanistic challenges as they work to copy both strands of DNA at the same time, and many years of classic biochemical studies have worked out how many of these challenges are overcome. However, in recent years, the use of single-molecule biophysical techniques has begun to challenge many aspects of the elegant textbook view of replisome function. In this project, we will develop and use a suite of new single-molecule techniques to define how the bacterial replisome really works. By doing so, we will work out how synthesis of the two DNA strands in different directions at the same time is coupled together and how timing mechanisms work.

Funding Amount 778790.999999999

Funding Scheme Discovery Projects