Data

Differential transcriptomic responses to heat in wild-type and heat-evolved Symbiodiniaceae in adult P. daedalea

Australian Ocean Data Network
Australian Institute of Marine Science (AIMS)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/ba6c9c47-a2a8-4015-b006-14e16292b210&rft.title=Differential transcriptomic responses to heat in wild-type and heat-evolved Symbiodiniaceae in adult P. daedalea&rft.identifier=https://apps.aims.gov.au/metadata/view/ba6c9c47-a2a8-4015-b006-14e16292b210&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Adult Platygyra daedalea colonies were chemically bleached and inoculated with Cladocopium proliferum strains (WT10, SS1, SS8). Three months after inoculation, the newly estbalished symbioses were subjected to a heat stress experiment. Corals were split between ambient (27°C) and elevated (32.2°C) temperature treatments. After 4.9 eDHWs of accumulated heat stress, samples were taken from both temperature treatments for RNA sequencing. Following total RNA extraction, mRNA libraries were prepared and sequenced at Ramaciotti Centre for Genomics. A differential expression analyis in response to heat was then performed on coral host and Symbiodiniaceae gene expression data.Maintenance and Update Frequency: notPlannedStatement: Sequencing was performed on an Illumina NovaSeq 6000 platform, using two lanes of a S4 flow cell.&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=147.05617815256122; southlimit=-19.268617069816585; eastlimit=147.05617815256122; northlimit=-19.268617069816585&rft.coverage=westlimit=147.05617815256122; southlimit=-19.268617069816585; eastlimit=147.05617815256122; northlimit=-19.268617069816585&rft_rights= http://creativecommons.org/licenses/by/3.0/au/&rft_rights=http://i.creativecommons.org/l/by/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2023). Differential transcriptomic responses to heat in wild-type and heat-evolved Symbiodiniaceae in adult P. daedalea. https://apps.aims.gov.au/metadata/view/ba6c9c47-a2a8-4015-b006-14e16292b210, accessed[date-of-access].&rft_rights=Creative Commons Attribution 3.0 Australia License http://creativecommons.org/licenses/by/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2023). Differential transcriptomic responses to heat in wild-type and heat-evolved Symbiodiniaceae in adult P. daedalea. https://apps.aims.gov.au/metadata/view/ba6c9c47-a2a8-4015-b006-14e16292b210, accessed[date-of-access]".

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Brief description

Adult Platygyra daedalea colonies were chemically bleached and inoculated with Cladocopium proliferum strains (WT10, SS1, SS8). Three months after inoculation, the newly estbalished symbioses were subjected to a heat stress experiment. Corals were split between ambient (27°C) and elevated (32.2°C) temperature treatments. After 4.9 eDHWs of accumulated heat stress, samples were taken from both temperature treatments for RNA sequencing. Following total RNA extraction, mRNA libraries were prepared and sequenced at Ramaciotti Centre for Genomics. A differential expression analyis in response to heat was then performed on coral host and Symbiodiniaceae gene expression data.

Lineage

Maintenance and Update Frequency: notPlanned
Statement: Sequencing was performed on an Illumina NovaSeq 6000 platform, using two lanes of a S4 flow cell.

Notes

Credit
Scharfenstein, HJ. Australian Institute of Marine Scienc (AIMS) & The University of Melbourne
Credit
Buerger, P. Macquarie University
Credit
Nitschke, MR. AIMS & Victoria University of Wellington
Credit
Peplow, LM. AIMS
Credit
Chan, WY. The University of Melbourne
Credit
van Oppen, MJH. AIMS & The University of Melbourne
Credit
Reef Restoration and Adaptation Program (RRAP), Australia
Credit
Paul G. Allen Family Foundation, USA
Credit
ARC Laureate Fellowship, No. FL180100036, Australian Research Council, Australia
Credit
Environmental Microbiology Research Initiative (EMRI) Research Grant, Australia

Modified: 10 08 2024

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147.05618,-19.26862

147.05617815256,-19.268617069817

text: westlimit=147.05617815256122; southlimit=-19.268617069816585; eastlimit=147.05617815256122; northlimit=-19.268617069816585

Subjects
oceans |

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Other Information
Input dataset for R code

uri : https://api.aims.gov.au/data-v2.0/ba6c9c47-a2a8-4015-b006-14e16292b210/files/RNAseq dataset.xlsx

Gene count matrix - photosymbiont

uri : https://api.aims.gov.au/data-v2.0/ba6c9c47-a2a8-4015-b006-14e16292b210/files/gene_counts_matrix_cprol.txt.zip

Gene count matrix - host

uri : https://api.aims.gov.au/data-v2.0/ba6c9c47-a2a8-4015-b006-14e16292b210/files/gene_counts_matrix_pdae.txt.zip

Identifiers
  • global : ba6c9c47-a2a8-4015-b006-14e16292b210
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