Differential regulation of two modes of exocytosis by protein phosphatases [ 2004 - 2006 ]

Research Grant

Researchers: A/Pr Alistair Sim (Principal investigator) ,  E/Pr John Rostas

Brief description The release of signals from nerve endings (exocytosis) is of fundamental importance to nervous system function. The recent recognition that nerve cells can release transmitter by at least two distinct modes of exocytosis has led to the need for a deeper understanding of the mechanisms that regulate exocytosis. Our identification of the key role played by two enzymes, PP2A and PP2B, represents a major insight into the molecular mechansisms regulating this process. The experiments will lead to the identification of how PP2A and PP2B are regulated at the molecular level and the targets that are important in the control of exocytosis. The project will also develop new tools to specifically manipulate the two modes of exocytosis in order to understand their contribution to normal and pathological neurotransmission. Strong stimulation of exocytosis is associated with learning and memory in normal brain and neuronal damage under certain pathological conditions. Since switching from 1 mode of exocytosis to another is also induced by strong stimulation, an understanding of the molecular mechanisms that control the different modes of exocytosis may eventually lead to clinical applications.

Funding Amount $AUD 399,750.00

Funding Scheme NHMRC Project Grants

Notes Standard Project Grant