Data

Determination of pteridines and flavins in seawater from Davies Reef, Great Barrier Reef

Australian Ocean Data Network
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/b98047dc-6c52-427e-ba3e-dcb762adff31&rft.title=Determination of pteridines and flavins in seawater from Davies Reef, Great Barrier Reef&rft.identifier=https://apps.aims.gov.au/metadata/view/b98047dc-6c52-427e-ba3e-dcb762adff31&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Individual pteridines and flavins from marine waters were analysed at picomolar concentrations. Samples were concentrated by adsorption on bonded-phase, C18 silica and Florisil Sep-Pak extraction columns connected in series.Sample extracts were analysed by reverse-phase liquid chromatography with fluorometric detection enabling quantitative identification of pteridines and flavins from reef waters at Davies Reef on Great Barrier Reef. Retention times, percent recovery, relative molar fluorescence and detection limits were recorded for each component.The application of these methods to the measurement of pterin and flavin excretion from several reef-dwelling, benthic organisms (the coral Acropora sp. and sponge Phyllopsongia sp.) was tested in some experiments. To examine the presence of flavin in pteridine micronutirients in coral reef waters. Pteridines: 6-biopterin, 6-hydroxymethylpterin, isoxanthopterin, Lumazine, Lumazine-6-carboxylic acid, pterin, pterin-6-carboxylic acid, xanthopterin.Flavins: lumiflavin, lumichrome, riboflavin, riboflavin-5-phosphate.Maintenance and Update Frequency: notPlannedStatement: Statement: Methods are outlined in the published paper.&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=147.623202; southlimit=-18.849678; eastlimit=147.671809; northlimit=-18.798919&rft.coverage=westlimit=147.623202; southlimit=-18.849678; eastlimit=147.671809; northlimit=-18.798919&rft_rights= http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Determination of pteridines and flavins in seawater from Davies Reef, Great Barrier Reef. https://apps.aims.gov.au/metadata/view/b98047dc-6c52-427e-ba3e-dcb762adff31, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Determination of pteridines and flavins in seawater from Davies Reef, Great Barrier Reef. https://apps.aims.gov.au/metadata/view/b98047dc-6c52-427e-ba3e-dcb762adff31, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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Brief description

Individual pteridines and flavins from marine waters were analysed at picomolar concentrations. Samples were concentrated by adsorption on bonded-phase, C18 silica and Florisil Sep-Pak extraction columns connected in series.Sample extracts were analysed by reverse-phase liquid chromatography with fluorometric detection enabling quantitative identification of pteridines and flavins from reef waters at Davies Reef on Great Barrier Reef. Retention times, percent recovery, relative molar fluorescence and detection limits were recorded for each component.The application of these methods to the measurement of pterin and flavin excretion from several reef-dwelling, benthic organisms (the coral Acropora sp. and sponge Phyllopsongia sp.) was tested in some experiments. To examine the presence of flavin in pteridine micronutirients in coral reef waters. Pteridines: 6-biopterin, 6-hydroxymethylpterin, isoxanthopterin, Lumazine, Lumazine-6-carboxylic acid, pterin, pterin-6-carboxylic acid, xanthopterin.Flavins: lumiflavin, lumichrome, riboflavin, riboflavin-5-phosphate.

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Maintenance and Update Frequency: notPlanned
Statement: Statement: Methods are outlined in the published paper.

Notes

Credit
Dunlap, Walter C, Dr (Principal Investigator)

Modified: 10 08 2024

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147.67181,-18.79892 147.67181,-18.84968 147.6232,-18.84968 147.6232,-18.79892 147.67181,-18.79892

147.6475055,-18.8242985

text: westlimit=147.623202; southlimit=-18.849678; eastlimit=147.671809; northlimit=-18.798919

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Other Information
Determination of pteridines and flavins in seawater by reverse-phase, high-performance liquid chromatography with fluorometric detection: Dunlap WC and Susic M (1985) Determination of pteridines and flavins in seawater by reverse-phase, high-performance liquid chromatography with fluorometric detection. Marine Chemistry 17: 185-198.

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  • global : b98047dc-6c52-427e-ba3e-dcb762adff31
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