Data

Data for flow cytometry, FlowCam, and microscopy comparison experiment

Australian Antarctic Division
Deppeler, S., Hancock, A. and Davidson, A. ; DEPPELER, STACY LOUISE ; HANCOCK, ALYCE ; DAVIDSON, ANDREW TIMOTHY
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.aad.gov.au/metadata/AAS_4026_Variance_Experiment&rft.title=Data for flow cytometry, FlowCam, and microscopy comparison experiment&rft.identifier=http://data.aad.gov.au/metadata/AAS_4026_Variance_Experiment&rft.publisher=Australian Antarctic Data Centre&rft.description=This data set was collected from two minicosm experiments conducted at Davis Station, Antarctica.1. Variance experiment - 2013/14 summer season2. Ocean acidification experiment - 2014/15 summer seasonIt includes:- description of methods for all data collection and analyses.- environmental data logged throughout the experiment; nutrients, temperature, light climate.- flow cytometry counts; autotrophic cells, heterotrophic nanoflagellates, and prokaryotes.- FlowCam counts; individual phytoplankton species data.- microscopy counts; individual phytoplankton species data.Context from the public summary for project 4026:Manmade CO2 has increased ocean acidity by 30% and it is projected to rise 300% by 2100. Antarctic waters will be amongst the earliest and most severely affected by this increase. Microbes are the base of the marine food chain and primary drivers of the biological pump. This project will incubate natural communities of Antarctic marine microbes in minicosms at a range of CO2 concentrations to quantify changes in their structure and function, the physiological responses that drive these changes, and provide input to models that predict effects on biogeochemical cycles and Antarctic food webs.Progress Code: completedStatement: The minicosm experimental design measured the microbial community growth in six unreplicated fCO2 treatments. Therefore, sub-samples from each minicosm were within-treatment pseudoreplicates and thus, results of statistical analysis must be interpreted conservatively.&rft.creator=Deppeler, S., Hancock, A. and Davidson, A. &rft.creator=DEPPELER, STACY LOUISE &rft.creator=HANCOCK, ALYCE &rft.creator=DAVIDSON, ANDREW TIMOTHY &rft.date=2018&rft.coverage=westlimit=77.89; southlimit=-68.59; eastlimit=77.99; northlimit=-68.56&rft.coverage=westlimit=77.89; southlimit=-68.59; eastlimit=77.99; northlimit=-68.56&rft_rights=These data are publicly available for download from the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4026_Variance_Experiment when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=biota&rft_subject=oceans&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON > PHYTOPLANKTON&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA&rft_subject=EARTH SCIENCE > CLIMATE INDICATORS > ATMOSPHERIC/OCEAN INDICATORS > OCEAN ACIDIFICATION&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > FLAGELLATES&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > FLAGELLATES > DINOFLAGELLATES&rft_subject=OCEAN ACIDIFICATION&rft_subject=FLOWCAM&rft_subject=FLOW CYTOMETRY&rft_subject=MICROSCOPES&rft_subject=FIELD INVESTIGATION&rft_subject=LABORATORY&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=AMD&rft_subject=GEOGRAPHIC REGION > POLAR&rft_subject=OCEAN > SOUTHERN OCEAN > Prydz Bay&rft_subject=CONTINENT > ANTARCTICA > Davis Station&rft.type=dataset&rft.language=English Access the data
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Licence & Rights:

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CC-BY

Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

These data are publicly available for download from the provided URL.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4026_Variance_Experiment when using these data.

This metadata record is publicly available.

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Full description

This data set was collected from two minicosm experiments conducted at Davis Station, Antarctica.
1. Variance experiment - 2013/14 summer season
2. Ocean acidification experiment - 2014/15 summer season

It includes:
- description of methods for all data collection and analyses.
- environmental data logged throughout the experiment; nutrients, temperature, light climate.
- flow cytometry counts; autotrophic cells, heterotrophic nanoflagellates, and prokaryotes.
- FlowCam counts; individual phytoplankton species data.
- microscopy counts; individual phytoplankton species data.

Context from the public summary for project 4026:
Manmade CO2 has increased ocean acidity by 30% and it is projected to rise 300% by 2100. Antarctic waters will be amongst the earliest and most severely affected by this increase. Microbes are the base of the marine food chain and primary drivers of the biological pump. This project will incubate natural communities of Antarctic marine microbes in minicosms at a range of CO2 concentrations to quantify changes in their structure and function, the physiological responses that drive these changes, and provide input to models that predict effects on biogeochemical cycles and Antarctic food webs.

Lineage

Progress Code: completed
Statement: The minicosm experimental design measured the microbial community growth in six unreplicated fCO2 treatments. Therefore, sub-samples from each minicosm were within-treatment pseudoreplicates and thus, results of statistical analysis must be interpreted conservatively.

Notes

Purpose
The purpose of this data set is to compare the results of three commonly used techniques for microbial community composition and abundance counts.

Data time period: 2013-11-01 to 2015-02-28

This dataset is part of a larger collection

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77.99,-68.56 77.99,-68.59 77.89,-68.59 77.89,-68.56 77.99,-68.56

77.94,-68.575

text: westlimit=77.89; southlimit=-68.59; eastlimit=77.99; northlimit=-68.56

Subjects
AMD | AMD/AU | CEOS | CONTINENT > ANTARCTICA > Davis Station | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > FLAGELLATES | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > FLAGELLATES > DINOFLAGELLATES | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON > PHYTOPLANKTON | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON | EARTH SCIENCE > CLIMATE INDICATORS > ATMOSPHERIC/OCEAN INDICATORS > OCEAN ACIDIFICATION | FIELD INVESTIGATION | FLOW CYTOMETRY | FLOWCAM | GEOGRAPHIC REGION > POLAR | LABORATORY | MICROSCOPES | OCEAN > SOUTHERN OCEAN > Prydz Bay | OCEAN ACIDIFICATION | biota | oceans |

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Other Information
Download a copy of the dataset (GET DATA > DIRECT DOWNLOAD)

uri : https://data.aad.gov.au/eds/4809/download

Public information for AAS project AAS_4026 (PROJECT HOME PAGE)

uri : https://projects.aad.gov.au/report_project_public.cfm?project_no=AAS_4026

Citation reference for this metadata record and dataset. (VIEW RELATED INFORMATION)

uri : https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4026_Variance_Experiment

Identifiers
  • global : AAS_4026_Variance_Experiment