crassphage_genomes_colabfold.zip

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CrAssphages are one of the most abundant phages in the human microbiome, and there are close to 600 genomes identified computationally, but only four have been successfully cultured. Here, we identify and characterize three novel crAssphage species isolated from wastewater and infecting the bacterial host Bacteroides cellulosilyticus WH2. The novel species span two families and three genera which we have named Kehishuvirus winsdale (Bc01), Kolpuevirus frurule (Bc03), and Rudgehvirus redwords (Bc11). These three new species have podovirus-like morphology, and their approximately 100 kb genome has a consistent arrangement with known crAssphages.  

Crystal structure models of all 103 proteins from Kehishuvirus winsdale (Bc01), Kolpuevirus frurule (Bc03), and Rudgehvirus redwords (Bc11) were generated using Colabfold version 1.4.