Data

Chevron_GAB Greentail prawn RNA sequences

Commonwealth Scientific and Industrial Research Organisation
Armstrong, Emily ; Miller, Adam ; Mondon, Julie ; Greenfield, Paul ; Stephenson, Sarah ; Tan, Mun Hua ; Gan, Han Ming ; Hook, Sharon
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25919/5c12fe7dae432&rft.title=Chevron_GAB Greentail prawn RNA sequences&rft.identifier=https://doi.org/10.25919/5c12fe7dae432&rft.publisher=Commonwealth Scientific and Industrial Research Organisation&rft.description=Raw RNA sequences from 27 individual greentail prawns (Metapenaeus bennettae) collected from St Vincent Gulf in South Australia in February 2016. Prawns were placed in tanks with either clean sediment or sediment spiked with 2 mg/kg of light-weight crude oil and exposed for 24 and 90 hrs before being transferred to clean sediment tanks for a further 90 hrs. Five prawns were removed from each tank at each of the different time periods. The aprrox. 10 mg of hepatopancreas was removed and place in RNAlater before freezing at -80. RNA libraries were prepared and sequenced by the Ramaciotti Centre (Sydeny, Australia), where libraries were prepared using 1 µg RNA from 27 individuals following Illumina protocols, and sequenced on two HiSeq2500 100bp single-end lanes in accordance with the manufacturer’s protocols. &rft.creator=Armstrong, Emily &rft.creator=Miller, Adam &rft.creator=Mondon, Julie &rft.creator=Greenfield, Paul &rft.creator=Stephenson, Sarah &rft.creator=Tan, Mun Hua &rft.creator=Gan, Han Ming &rft.creator=Hook, Sharon &rft.date=2018&rft.edition=v1&rft_rights=Creative Commons Attribution 4.0 International Licence https://creativecommons.org/licenses/by/4.0/&rft_rights=Data is accessible online and may be reused in accordance with licence conditions&rft_rights=All Rights (including copyright) CSIRO 2018.&rft_subject=Greentail prawn&rft_subject=GAB&rft_subject=crude oil&rft_subject=RNA-Seq data&rft_subject=Other environmental sciences not elsewhere classified&rft_subject=Other environmental sciences&rft_subject=ENVIRONMENTAL SCIENCES&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution 4.0 International Licence
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Data is accessible online and may be reused in accordance with licence conditions

All Rights (including copyright) CSIRO 2018.

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Raw RNA sequences from 27 individual greentail prawns (Metapenaeus bennettae) collected from St Vincent Gulf in South Australia in February 2016. Prawns were placed in tanks with either clean sediment or sediment spiked with 2 mg/kg of light-weight crude oil and exposed for 24 and 90 hrs before being transferred to clean sediment tanks for a further 90 hrs. Five prawns were removed from each tank at each of the different time periods. The aprrox. 10 mg of hepatopancreas was removed and place in RNAlater before freezing at -80. RNA libraries were prepared and sequenced by the Ramaciotti Centre (Sydeny, Australia), where libraries were prepared using 1 µg RNA from 27 individuals following Illumina protocols, and sequenced on two HiSeq2500 100bp single-end lanes in accordance with the manufacturer’s protocols.

Available: 2018-12-14

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Subjects
Environmental Sciences | GAB | Greentail prawn | Other Environmental Sciences | Other Environmental Sciences Not Elsewhere Classified | RNA-Seq data | crude oil |

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