Characterisation of a novel direct electrochemical chip as a biosensor and tool for studying redox-sensitive proteins [ 2004 - 2005 ]

Research Grant

Researchers: A/Pr Lisandra Martin (Principal investigator) ,  Prof Raymond Rodgers

Brief description Biosensors use biomolecules to detect a chemical event. They are becoming important for the rapid and reliable measurement of the concentrations of molecules in fluids. In human medicine they will be of great use to general practitioners and patients for instantaneous read outs of concentrations of many different biological molecules. How well a biosensor responds depends on the method in which the biomolecule is immobilised to a surface and the signal detected. We have made a significant advance in biosensing capabilities using a recombinant protein (thioredoxin) and demonstrated the improvement that is possible by (i) immobilising the protein in a highly oriented way and (ii) using a sensitive electrical signal to monitor the response. Here we will undertake more comprehensive testing by extending the number of proteins to include the 4 major classes of redox-sensitive biomolecules (proteins) in the body. This will enable us to establish the broad application of our methods and substantially improve our ability to commercialize our discoveries.

Funding Amount $AUD 144,500.00

Funding Scheme NHMRC Development Grants

Notes Development Grant