Data

BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006

Australian Antarctic Data Centre
THOMSON, PAUL GERARD
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.4225/15/59890669dda1a&rft.title=BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006&rft.identifier=10.4225/15/59890669dda1a&rft.publisher=Australian Antarctic Data Centre&rft.description=This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were assessed for transect 11. Between 4 and 12 depths were sampled for marine microbes and concentrations were assessed using FACScan flowcytometer. Phytoplankton were identified and counted based on the autofluorescense of chlorophyll a when excited by the 488 nm laser of the FACScan. Protozoa were identified and counted after staining with the acid vacuole stain Lysotracker Green. Total bacteria were identified and counted using the cell permeant SYTO 13 nucleic stain. Metabolically active bacteria were identified and counted after staining for intracellular esterases with the esterase stain 6CFDA. The fields in this dataset are: Latitude Longitude Transect Number CTD number, flow file Depth (m) Total bacteria (per millilitre) Active bacteria (per millilitre) Dead bacteria (per millilitre) Protozoa (per millilitre) Phytoplankton (per millilitre) This work was completed as part of ASAC project 40 (ASAC_40).&rft.creator=THOMSON, PAUL GERARD &rft.date=2006&rft.coverage=northlimit=-59.79757; southlimit=-69.20696; westlimit=29.99776; eastLimit=80.01055; projection=WGS84&rft.coverage=northlimit=-59.79757; southlimit=-69.20696; westlimit=29.99776; eastLimit=80.01055; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_flow_cytometry when using these data.&rft_subject=biota&rft_subject=oceans&rft_subject=PROTISTS&rft_subject=EARTH SCIENCE&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=BACTERIA/ARCHAEA&rft_subject=PHYTOPLANKTON&rft_subject=BIOSPHERE&rft_subject=AQUATIC ECOSYSTEMS&rft_subject=PLANKTON&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC&rft_subject=BROKE-West&rft_subject=Flow cytometry&rft_subject=bacteria&rft_subject=FACScan Flow Cytometer&rft_subject=Latitude&rft_subject=Longitude&rft_subject=Transect Number&rft_subject=CTD&rft_subject=Depth&rft_subject=Total bacteria&rft_subject=Active bacteria&rft_subject=Dead bacteria&rft_subject=Protozoa&rft_subject=Phytoplankton&rft_subject=FLOW CYTOMETRY&rft_subject=SHIPS&rft_subject=R/V AA > R/V Aurora Australis&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=CONTINENT > ANTARCTICA&rft_subject=GEOGRAPHIC REGION > POLAR&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_flow_cytometry when using these data.

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Brief description

This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were assessed for transect 11.

Between 4 and 12 depths were sampled for marine microbes and concentrations were assessed using FACScan flowcytometer. Phytoplankton were identified and counted based on the autofluorescense of chlorophyll a when excited by the 488 nm laser of the FACScan. Protozoa were identified and counted after staining with the acid vacuole stain Lysotracker Green. Total bacteria were identified and counted using the cell permeant SYTO 13 nucleic stain. Metabolically active bacteria were identified and counted after staining for intracellular esterases with the esterase stain 6CFDA.

The fields in this dataset are:

Latitude
Longitude
Transect Number
CTD number, flow file
Depth (m)
Total bacteria (per millilitre)
Active bacteria (per millilitre)
Dead bacteria (per millilitre)
Protozoa (per millilitre)
Phytoplankton (per millilitre)

This work was completed as part of ASAC project 40 (ASAC_40).

Issued: 2006-05-19

Data time period: 2006-01-10 to 2006-02-27

This dataset is part of a larger collection

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80.01055,-59.79757 80.01055,-69.20696 29.99776,-69.20696 29.99776,-59.79757 80.01055,-59.79757

55.004155,-64.502265

text: northlimit=-59.79757; southlimit=-69.20696; westlimit=29.99776; eastLimit=80.01055; projection=WGS84

Subjects
AQUATIC ECOSYSTEMS | Active bacteria | BACTERIA/ARCHAEA | BIOLOGICAL CLASSIFICATION | BIOSPHERE | BROKE-West | CONTINENT > ANTARCTICA | CTD | Dead bacteria | Depth | EARTH SCIENCE | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC | FACScan Flow Cytometer | FLOW CYTOMETRY | Flow cytometry | GEOGRAPHIC REGION > POLAR | Latitude | Longitude | OCEAN > SOUTHERN OCEAN | PHYTOPLANKTON | PLANKTON | PROTISTS | Phytoplankton | Protozoa | R/V AA > R/V Aurora Australis | SHIPS | Total bacteria | Transect Number | bacteria | biota | oceans |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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