Data

Broad genotypic variation of a genetic diverse Brassica napus population

The University of Western Australia
Chen, Sheng ; Cowling, Wallace ; Siddique, Kadambot
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26182/80cs-xm11&rft.title=Broad genotypic variation of a genetic diverse Brassica napus population&rft.identifier=10.26182/80cs-xm11&rft.publisher=The University of Western Australia&rft.description=While the phenotype of 322 Brassica napus lines were screened for heat tolerance using a prototype heat screening facility, their genotypic variation was investigated. Single nucleotide polymorphism (SNP) genotyping was performed using the Illumina Infinium 60K Brassica SNP array containing 52,157 SNP markers from the A and C sub-genomes in B. napus. Hybridization protocols were performed according to the manufacturer's instructions for all samples and the lined data was visualized and exported using Genome Studio v2.0.4 software (Illumina Inc., San Diego CA, USA). Then, SNPs with ≥ 20% missing data and monomorphic were omitted. The SNP data for the association analyses were exported in HapMap format, where the line can be either homozygous, heterozygous or missed call (NN). The SNPs were mapped to the Darmor-bzh v4 reference sequence (Chalhoub et al. 2014) via BLAST of the SNP probe sequences. &rft.creator=Chen, Sheng &rft.creator=Cowling, Wallace &rft.creator=Siddique, Kadambot &rft.date=2023&rft_subject=GRDC&rft_subject=UWA1905-007RTX&rft_subject=heat stress tolerance&rft_subject=Climate change&rft_subject=Genetics and breeding&rft_subject=plant physiology under abiotic stress&rft_subject=brassica napus&rft_subject=Canola&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Access:

Restrictions apply

Full description

While the phenotype of 322 Brassica napus lines were screened for heat tolerance using a prototype heat screening facility, their genotypic variation was investigated. Single nucleotide polymorphism (SNP) genotyping was performed using the Illumina Infinium 60K Brassica SNP array containing 52,157 SNP markers from the A and C sub-genomes in B. napus. Hybridization protocols were performed according to the manufacturer's instructions for all samples and the lined data was visualized and exported using Genome Studio v2.0.4 software (Illumina Inc., San Diego CA, USA). Then, SNPs with ≥ 20% missing data and monomorphic were omitted. The SNP data for the association analyses were exported in HapMap format, where the line can be either homozygous, heterozygous or missed call (NN). The SNPs were mapped to the Darmor-bzh v4 reference sequence (Chalhoub et al. 2014) via BLAST of the SNP probe sequences.

Created: 2020-04 to 2023-04

Issued: 2023-04

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related Organisations

Related People

Related Grants and Projects

Subjects
Canola | Climate change | GRDC | Genetics and breeding | UWA1905-007RTX | brassica napus | heat stress tolerance | plant physiology under abiotic stress |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Identifiers
Similar data you may be interested in: