Data

BEAGLE 2003, Leg 5, Cape Town (South Africa) - Fremantle (Australia), HPLC and Ocean Colour Data

Australian Ocean Data Network
CSIRO O&A, Information & Data Centre (Point of contact) Clementson, Lesley (Point of contact) Lenton, Andrew (Associated with)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/bc1b3741-e33d-5039-e044-00144f7bc0f4&rft.title=BEAGLE 2003, Leg 5, Cape Town (South Africa) - Fremantle (Australia), HPLC and Ocean Colour Data&rft.identifier=Anzlic Identifier: ANZCW0306008031&rft.publisher=Australian Ocean Data Network&rft.description=The Japan Agency for Marine-Earth Sciences and Technology (JAMSTEC) sponsored this research cruise - a circumnavigation of the Southern hemisphere using the World Ocean Circulation Experiment (WOCE) Hydrographic Program (WHP) lines in the southern hemisphere to celebrate the 30th anniversary of its establishment. The research cruise was named BEAGLE 2003 (Blue EArth GLobal Expedition 2003) and was carried out on the RV MIRAI. This is a subset of the Beagle 2003 data set and contains the HPLC pigment and particulate absorption coefficient data collected on leg 5, between Cape Town, South Africa and Fremantle, Australia from the 11 Dec 2003 to 20 Jan 2004. The data can be used for Ocean Colour sensor validation. Parameters measured on the leg are the concentration of chlorophyll and carotenoid pigments and the absorption coefficent for particulate (a/p) components of the water column. Samples were collected at 43 stations. The data was used primarily to validate ocean colour sensors MERIS, MODIS and SeaWIFs and the SST sensor AATSR. Samples were collected and analysed for pigments and absorption coefficient, particulate.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the RV MIRAI and stored under cool and dark conditions until filtering took place onboard the vessel. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory, CSIRO, Hobart, Tasmania, Australia. Pigment analysis; between 1-2.5 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a,chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption; between 1-2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere&rft.creator=Anonymous&rft.date=2008&rft.coverage=westlimit=28.238; southlimit=-34.575; eastlimit=113.75; northlimit=-19.987&rft.coverage=westlimit=28.238; southlimit=-34.575; eastlimit=113.75; northlimit=-19.987&rft.coverage=uplimit=0; downlimit=0&rft.coverage=uplimit=0; downlimit=0&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=CHLOROPHYLL&rft_subject=EARTH SCIENCE&rft_subject=OCEANS&rft_subject=OCEAN CHEMISTRY&rft_subject=PIGMENTS&rft_subject=OCEAN COLOR&rft_subject=OCEAN OPTICS&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Global / Oceans | Indian Ocean&rft_subject=WfO Biogeochemistry&rft_subject=Remote Sensing 2004-2007&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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The Japan Agency for Marine-Earth Sciences and Technology (JAMSTEC) sponsored this research cruise - a circumnavigation of the Southern hemisphere using the World Ocean Circulation Experiment (WOCE) Hydrographic Program (WHP) lines in the southern hemisphere to celebrate the 30th anniversary of its establishment. The research cruise was named "BEAGLE 2003" (Blue EArth GLobal Expedition 2003) and was carried out on the RV MIRAI. This is a subset of the Beagle 2003 data set and contains the HPLC pigment and particulate absorption coefficient data collected on leg 5, between Cape Town, South Africa and Fremantle, Australia from the 11 Dec 2003 to 20 Jan 2004. The data can be used for Ocean Colour sensor validation. Parameters measured on the leg are the concentration of chlorophyll and carotenoid pigments and the absorption coefficent for particulate (a/p) components of the water column. Samples were collected at 43 stations. The data was used primarily to validate ocean colour sensors MERIS, MODIS and SeaWIFs and the SST sensor AATSR. Samples were collected and analysed for pigments and absorption coefficient, particulate.

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Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the RV MIRAI and stored under cool and dark conditions until filtering took place onboard the vessel. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory, CSIRO, Hobart, Tasmania, Australia. Pigment analysis; between 1-2.5 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a,chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption; between 1-2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere

Notes

Credit
Ian Barton, The Japan Agency for Marine-Earth Sciences and Technology (JAMSTEC) (sponsor of research cruise)
Credit
Lesley Clementson

Data time period: 2003-12-11 to 2004-01-20

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113.75,-19.987 113.75,-34.575 28.238,-34.575 28.238,-19.987 113.75,-19.987

70.994,-27.281

text: westlimit=28.238; southlimit=-34.575; eastlimit=113.75; northlimit=-19.987

text: uplimit=0; downlimit=0

Subjects
Chlorophyll | CTD | CTDs (Conductivity-Temperature-Depth Profilers) | EARTH SCIENCE | Fluorometers | Global / Oceans | Indian Ocean | Niskin Bottles | OCEAN CHEMISTRY | Ocean Color | OCEAN OPTICS | OCEANS | Pigments | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Remote Sensing 2004-2007 | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Data Link)

uri : https://aesop.csiro.au/

BEAGLE 2003 home page (Data Link)

uri : https://www.jamstec.go.jp/beagle2003/en/preface.html

This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

uri : https://creativecommons.org/licenses/by/4.0/

Identifiers
  • global : bc1b3741-e33d-5039-e044-00144f7bc0f4
  • Local : Marlin Record Number: 8031
  • Local : Anzlic Identifier: ANZCW0306008031
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