Data

Archival chromatin sequencing in eastern water dragon museum specimens

Commonwealth Scientific and Industrial Research Organisation
Hahn, Erin ; Stiller, Jiri ; Alexander, Marina ; Grealy, Alicia ; Taylor, Jen ; Kent, Nicola ; Frere, Celine ; Holleley, Clare
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25919/eezr-h546&rft.title=Archival chromatin sequencing in eastern water dragon museum specimens&rft.identifier=https://doi.org/10.25919/eezr-h546&rft.publisher=Commonwealth Scientific and Industrial Research Organisation&rft.description=The data consist of 150 bp paired-end Illumina sequencing reads from chromatin conformation assay of 5 archival eastern water dragon (Intellagama lesueurii lesueurii) specimens and whole genome sequencing of three contemporary specimens. The archival specimens, collected between 1905 and 2001, were spirit-preserved and formalin-exposed. Archival liver tissue was dissected and subjected to MNase treatment heavily optimised for archival tissues. The contemporary specimens had been euthanised due to injury in accordance with Queensland Department of Environment and Sciences permit WA0038029 (Australian Ethics Committee number ANA20161, University of Sunshine Coast). From these fresh specimens, we extracted approximately 5 mg of liver tissue per specimen with the Qiagen AllPrep kit. The extracted or enriched DNA was then prepared for sequencing using an IDT xGEN Prism library prep kit.\n\nThe data were generated as part of a collaboration with Celine Frere at the University of Queensland.&rft.creator=Hahn, Erin &rft.creator=Stiller, Jiri &rft.creator=Alexander, Marina &rft.creator=Grealy, Alicia &rft.creator=Taylor, Jen &rft.creator=Kent, Nicola &rft.creator=Frere, Celine &rft.creator=Holleley, Clare &rft.date=2024&rft.edition=v3&rft.relation=https://doi.org/10.1038/s41467-024-50668-4&rft_rights=Creative Commons Attribution-Noncommercial 4.0 Licence https://creativecommons.org/licenses/by-nc/4.0/&rft_rights=Data is accessible online and may be reused in accordance with licence conditions&rft_rights=All Rights (including copyright) CSIRO 2023.&rft_subject=DNA&rft_subject=formaldehyde&rft_subject=formalin&rft_subject=genome&rft_subject=museum&rft_subject=museomics&rft_subject=epigenetics&rft_subject=museum epigenomics&rft_subject=chromatin accessibility&rft_subject=Epigenetics (incl. genome methylation and epigenomics)&rft_subject=Genetics&rft_subject=BIOLOGICAL SCIENCES&rft_subject=Critical heritage, museum and archive studies&rft_subject=Heritage, archive and museum studies&rft_subject=HISTORY, HERITAGE AND ARCHAEOLOGY&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution-Noncommercial 4.0 Licence
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Data is accessible online and may be reused in accordance with licence conditions

All Rights (including copyright) CSIRO 2023.

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Brief description

The data consist of 150 bp paired-end Illumina sequencing reads from chromatin conformation assay of 5 archival eastern water dragon (Intellagama lesueurii lesueurii) specimens and whole genome sequencing of three contemporary specimens. The archival specimens, collected between 1905 and 2001, were spirit-preserved and formalin-exposed. Archival liver tissue was dissected and subjected to MNase treatment heavily optimised for archival tissues. The contemporary specimens had been euthanised due to injury in accordance with Queensland Department of Environment and Sciences permit WA0038029 (Australian Ethics Committee number ANA20161, University of Sunshine Coast). From these fresh specimens, we extracted approximately 5 mg of liver tissue per specimen with the Qiagen AllPrep kit. The extracted or enriched DNA was then prepared for sequencing using an IDT xGEN Prism library prep kit.

The data were generated as part of a collaboration with Celine Frere at the University of Queensland.

Available: 2024-08-01

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Subjects
Biological Sciences | Critical Heritage, Museum and Archive Studies | DNA | Epigenetics (Incl. Genome Methylation and Epigenomics) | Genetics | History, Heritage and Archaeology | Heritage, Archive and Museum Studies | chromatin accessibility | epigenetics | formaldehyde | formalin | genome | museomics | museum | museum epigenomics |

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