Data

Antioxidant activity in extracts of marine organisms screened by microtiter plate assay

Australian Ocean Data Network
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/67feca49-df27-4cd2-a1d2-3b8f4387b175&rft.title=Antioxidant activity in extracts of marine organisms screened by microtiter plate assay&rft.identifier=https://apps.aims.gov.au/metadata/view/67feca49-df27-4cd2-a1d2-3b8f4387b175&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=A novel microtiter plate assay was developed to determine the total peroxyl radical-trapping activity of antioxidants extracted from marine organisms by measuring the inhibition rate of dye-substrate oxidation. The 96-well microtiter plate format afforded multisample throughput.Dihydrorhodamine-123 (DHR), dihydrofluorescein (DHR), and dichlorodihydrofluorescein (DCDHF) were used as reduced substrates for oxidation by peroxyl radicals generated from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH).The antioxidant inhibition rates for uric acid and trolox were measured on addition of antioxidant reagents to the aqueous portion of the reaction mixture. Other comparative data were obtained by adjusting concentrations of the reduced substrates or concentrations of the AAPH peroxyl initiator.Data were prepared for graphic presentation, and reaction rates were determined by linear regression using Kaleida-Graph software (Synergy Software, Reading, Pa.).The inaugural test examined 166 methanolic extracts obtained from a selection of coral reef organisms and commensal microbes cultured on marine agar. Extracts included the marine sponges Paramyxilla n.sp. and Lissodendoryx isodicryalis; and the halophilic algae, Dampier Salt #1A. To determine a simple, robust, and reliable method for screening antioxidant activity in extracts of marine organisms using microtiter plate technology to perform concurrent multi-sample analyses.To demonstrate a chromatographic strategy for examining the antioxidant composition of active extracts for novel lead selection, based on the principle of electrochemical detection.To design and test suitable procedures for shipboard analysis of freshly prepared extracts obtained from marine organisms.Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft_rights= http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Antioxidant activity in extracts of marine organisms screened by microtiter plate assay. https://apps.aims.gov.au/metadata/view/67feca49-df27-4cd2-a1d2-3b8f4387b175, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Antioxidant activity in extracts of marine organisms screened by microtiter plate assay. https://apps.aims.gov.au/metadata/view/67feca49-df27-4cd2-a1d2-3b8f4387b175, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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Brief description

A novel microtiter plate assay was developed to determine the total peroxyl radical-trapping activity of antioxidants extracted from marine organisms by measuring the inhibition rate of dye-substrate oxidation. The 96-well microtiter plate format afforded multisample throughput.Dihydrorhodamine-123 (DHR), dihydrofluorescein (DHR), and dichlorodihydrofluorescein (DCDHF) were used as reduced substrates for oxidation by peroxyl radicals generated from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH).The antioxidant inhibition rates for uric acid and trolox were measured on addition of antioxidant reagents to the aqueous portion of the reaction mixture. Other comparative data were obtained by adjusting concentrations of the reduced substrates or concentrations of the AAPH peroxyl initiator.Data were prepared for graphic presentation, and reaction rates were determined by linear regression using Kaleida-Graph software (Synergy Software, Reading, Pa.).The inaugural test examined 166 methanolic extracts obtained from a selection of coral reef organisms and commensal microbes cultured on marine agar. Extracts included the marine sponges Paramyxilla n.sp. and Lissodendoryx isodicryalis; and the halophilic algae, Dampier Salt #1A. To determine a simple, robust, and reliable method for screening antioxidant activity in extracts of marine organisms using microtiter plate technology to perform concurrent multi-sample analyses.To demonstrate a chromatographic strategy for examining the antioxidant composition of active extracts for novel lead selection, based on the principle of electrochemical detection.To design and test suitable procedures for shipboard analysis of freshly prepared extracts obtained from marine organisms.

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Maintenance and Update Frequency: notPlanned

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Credit
Dunlap, Walter C, Dr (Principal Investigator)

Modified: 09 08 2024

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Other Information
A microtiter plate assay for screening antioxidant activity in extracts of marine organisms: Dunlap WC, Llewellyn LE, Doyle JR and Yamamoto Y (2003) A microtiter plate assay for screening antioxidant activity in extracts of marine organisms. Marine Biotechnology 5: 294-301.

local : articleId=5835

A rapid high-throughput kinetic assay for screening antioxidant activity in extracts of marine organisms: Dunlap WC, Llewellyn LE, Doyle JR and Yamamoto Y (2000) A rapid high-throughput kinetic assay for screening antioxidant activity in extracts of marine organisms. p. 47. In: IMBC 2000. Townsville, Australia 29 September - 4 October 2000. Program & Abstracts.

local : articleId=7954

Sunscreens, oxidative stress and antioxidant functions in marine organisms of the Great Barrier Reef: Dunlap WC, Shick JM and Yamamoto Y (1999) Sunscreens, oxidative stress and antioxidant functions in marine organisms of the Great Barrier Reef. Redox Reports 4: 301-306.

local : articleId=5621

A novel antioxidant derived from seaweed: Dunlap WC, Masaki K, Yamamoto Y, Larsen RM and Karube I (1998) A novel antioxidant derived from seaweed. pp. 33-35.. In: Le Gal Y and Halvorson H (eds) New developments in marine biotechnology. Proceedings of the 4th International Marine Botechnology Conference, September 22-29, 1997. Plenum Press. 343 p.

local : articleId=1616

Small-molecule antioxidants in marine organisms: antioxidant activity of mycosporine-glycine: Dunlap WC and Yamamoto Y (1995) Small-molecule antioxidants in marine organisms: antioxidant activity of mycosporine-glycine. Comparative Biochemistry and Physiology 112B: 105-114.

local : articleId=2624

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  • global : 67feca49-df27-4cd2-a1d2-3b8f4387b175
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