Data

Antarctic Krill Gonad mRNA Transcriptome

Australian Antarctic Data Centre
DEAGLE, BRUCE ; POLANOWSKI, ANDREA
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/5cd3c8fec9ad8&rft.title=Antarctic Krill Gonad mRNA Transcriptome&rft.identifier=10.26179/5cd3c8fec9ad8&rft.publisher=Australian Antarctic Data Centre&rft.description=RNA was extracted from pooled gonad tissues and tails of five sexually mature males and females, respectively, originating from the krill aquarium at the AAD in Tasmania, Australia. For RNA extractions, RNeasy mini kits (QIAGEN) were used and total RNA (8 micrograms each) was sent to Geneworks, South Australia (www.geneworks.com.au), for Illumina TruSeq 75 bp paired-end sequencing in two technical replica. Reads Yield Total Yield Krill_Male_sex_a_read1_sequence.txt 8,120,993 609,074,475 bases 1,218,148,950 bases Krill_Male_sex_a_read2_sequence.txt 8,120,993 609,074,475 bases Krill_Male_sex_b_read1_sequence.txt 10,465,586 784,918,950 bases 1,569,837,900 bases Krill_Male_sex_b_read2_sequence.txt 10,465,586 784,918,950 bases Krill_Male_tissue_a_read1_sequence.txt 7,867,804 590,085,300 bases 1,180,170,600 bases Krill_Male_tissue_a_read2_sequence.txt 7,867,804 590,085,300 bases Krill_Male_tissue_b_read1_sequence.txt 10,956,251 821,718,825 bases 1,793,118,450 bases Krill_Male_tissue_b_read2_sequence.txt 10,956,251 821,718,825 bases Krill_Female_sex_read1a_sequence.txt 29,447,654 2,208,574,050 bases 4,417,148,100 bases Krill_Female_sex_read2a_sequence.txt 29,447,654 2,208,574,050 bases Krill_Female_sex_read1b_sequence.txt 18,223,515 1,366,763,625 bases 2,733,527,250 bases Krill_Female_sex_read2b_sequence.txt 18,223,515 1,366,763,625 bases The insert size for these libraries is approx 160bp.&rft.creator=DEAGLE, BRUCE &rft.creator=POLANOWSKI, ANDREA &rft.date=2019&rft.coverage=northlimit=-42.86485; southlimit=-43.09623; westlimit=147.16809; eastLimit=147.46326; projection=WGS84&rft.coverage=northlimit=-42.86485; southlimit=-43.09623; westlimit=147.16809; eastLimit=147.46326; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_AAS_4015_Krill_Gonad_Transriptome when using these data.&rft_subject=biota&rft_subject=oceans&rft_subject=EUPHAUSIIDS (KRILL)&rft_subject=EARTH SCIENCE&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=ANIMALS/INVERTEBRATES&rft_subject=ARTHROPODS&rft_subject=CRUSTACEANS&rft_subject=ANTARCTIC KRILL&rft_subject=TRANSCRIPTOME&rft_subject=MRNA&rft_subject=ADS > Automated DNA Sequencer&rft_subject=LABORATORY&rft_subject=GEOGRAPHIC REGION > POLAR&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=CONTINENT > ANTARCTICA&rft_subject=CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

view details

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_AAS_4015_Krill_Gonad_Transriptome when using these data.

Access:

Open view details

These data are publicly available for download from the provided URL

Brief description

RNA was extracted from pooled gonad tissues and tails of five sexually mature males and females, respectively, originating from the krill aquarium at the AAD in Tasmania, Australia. For RNA extractions, RNeasy mini kits (QIAGEN) were used and total RNA (8 micrograms each) was sent to Geneworks, South Australia (www.geneworks.com.au), for Illumina TruSeq 75 bp paired-end sequencing in two technical replica.

Reads Yield Total Yield
Krill_Male_sex_a_read1_sequence.txt 8,120,993 609,074,475 bases 1,218,148,950 bases
Krill_Male_sex_a_read2_sequence.txt 8,120,993 609,074,475 bases
Krill_Male_sex_b_read1_sequence.txt 10,465,586 784,918,950 bases 1,569,837,900 bases
Krill_Male_sex_b_read2_sequence.txt 10,465,586 784,918,950 bases

Krill_Male_tissue_a_read1_sequence.txt 7,867,804 590,085,300 bases 1,180,170,600 bases
Krill_Male_tissue_a_read2_sequence.txt 7,867,804 590,085,300 bases
Krill_Male_tissue_b_read1_sequence.txt 10,956,251 821,718,825 bases 1,793,118,450 bases
Krill_Male_tissue_b_read2_sequence.txt 10,956,251 821,718,825 bases

Krill_Female_sex_read1a_sequence.txt 29,447,654 2,208,574,050 bases 4,417,148,100 bases
Krill_Female_sex_read2a_sequence.txt 29,447,654 2,208,574,050 bases
Krill_Female_sex_read1b_sequence.txt 18,223,515 1,366,763,625 bases 2,733,527,250 bases
Krill_Female_sex_read2b_sequence.txt 18,223,515 1,366,763,625 bases

The insert size for these libraries is approx 160bp.

Issued: 2019-05-09

Data time period: 2012-07-01 to 2018-12-31

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related Data

Related Organisations

Related People

147.46326,-42.86485 147.46326,-43.09623 147.16809,-43.09623 147.16809,-42.86485 147.46326,-42.86485

147.315675,-42.98054

text: northlimit=-42.86485; southlimit=-43.09623; westlimit=147.16809; eastLimit=147.46326; projection=WGS84

Subjects
ADS > Automated DNA Sequencer | ANIMALS/INVERTEBRATES | ANTARCTIC KRILL | ARTHROPODS | BIOLOGICAL CLASSIFICATION | CONTINENT > ANTARCTICA | CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA | CRUSTACEANS | EARTH SCIENCE | EUPHAUSIIDS (KRILL) | GEOGRAPHIC REGION > POLAR | LABORATORY | MRNA | OCEAN > SOUTHERN OCEAN | TRANSCRIPTOME | biota | oceans |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Other Information

AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

Identifiers
Similar data you may be interested in: