A simple and innovative test for real-time detection of resistance in weeds

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File list: *A_simple_and_innovative_test_for_real_time_detection_of_resistance_in_weeds_R_Busi_21_2_23.xlsx *dataRecord_agar_test.pdf Methods for data generation and collection: *Preparation of agar containing various herbicides used for annual ryegrass control *Incubation of annual ryegrass seeds (susceptible and resistant populations) on agar under controlled and uncontrolled conditions *Assessment of germination and seedling growth on herbicide-containing agar *Assessment of a range of potential dormancy-breaking treatments *Measurement of herbicide concentration in agar after various storage times *Pot-based dose-response assays to compare results Data processing: Microsoft Excel; R (drc package); Graph Pad Prism Specific information needed to process data: N/A Standards and calibration information: N/A No. variables: 3 (herbicide treatment, incubation conditions, ryegrass population) Experimental conditions: ambient laboratory temperature and light or 25/15 C alternating temperatures with 12 h photoperiod of white LED light (300 umol/m2/s)