Data

A modular dCas9-based recruitment platform for combinatorial epigenome editing

The University of Western Australia
Swain, Tessa ; Pflueger, Christian ; Freytag, Saskia ; Poppe, Daniel ; Pflueger, Jahnvi ; Nguyen, Trung Viet ; Li, Ji Kevin ; Lister, Ryan
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26182/v862-2215&rft.title=A modular dCas9-based recruitment platform for combinatorial epigenome editing&rft.identifier=10.26182/v862-2215&rft.publisher=The University of Western Australia&rft.description=Chromatin immunoprecipitation sequencing (ChIP-seq) for histone modifications H3K4me3, H3K9me3 and H3K27me3 in HEK293T cells stably expressing dCas9-SSSavi and 6x sgRNA, and transfected with either DNMT3A+KRAB+EZH2 (D3A-K-E) or aGCN4-mCherry (non-catalytic control) for 72h, followed by flow cytometric enrichment of 200,000 cells positive for GFP (containing SSSavi components). WGBS was performed on HSB6G cells transfected with either D3A-K-E or GCN4-mCherry for 72h, followed by flow cytometric enrichment of 100,000 cells positive for GFP (containing SSSavi components). Genomic DNA was extracted and processed as detailed in the protocols section.&rft.creator=Swain, Tessa &rft.creator=Pflueger, Christian &rft.creator=Freytag, Saskia &rft.creator=Poppe, Daniel &rft.creator=Pflueger, Jahnvi &rft.creator=Nguyen, Trung Viet &rft.creator=Li, Ji Kevin &rft.creator=Lister, Ryan &rft.date=2024&rft.relation=http://research-repository.uwa.edu.au/en/publications/8b75c397-10ff-4504-a207-b80ecacb40d2&rft.type=dataset&rft.language=English Access the data
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Chromatin immunoprecipitation sequencing (ChIP-seq) for histone modifications H3K4me3, H3K9me3 and H3K27me3 in HEK293T cells stably expressing dCas9-SSSavi and 6x sgRNA, and transfected with either DNMT3A+KRAB+EZH2 (D3A-K-E) or aGCN4-mCherry (non-catalytic control) for 72h, followed by flow cytometric enrichment of 200,000 cells positive for GFP (containing SSSavi components). WGBS was performed on HSB6G cells transfected with either D3A-K-E or GCN4-mCherry for 72h, followed by flow cytometric enrichment of 100,000 cells positive for GFP (containing SSSavi components). Genomic DNA was extracted and processed as detailed in the protocols section.

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Ji Kevin Li (Creator)

Issued: 2024-01-17

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