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18S and COI sequence data from cross-lab reproducible metabarcoding study

Commonwealth Scientific and Industrial Research Organisation
Greenfield, Paul ; Cawthron Institute ; National Institute of Water and Atmospheric Research (NIWA) ; University of Otago ; University of Auckland ; Curtin University ; Macquarie University ; Moss Landing Marine Laboratory ; Department of Fisheries and Oceans ; McGill University ; University of Guelph ; University of Oviedo ; Klaipeda University
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25919/5j5x-0711&rft.title=18S and COI sequence data from cross-lab reproducible metabarcoding study&rft.identifier=https://doi.org/10.25919/5j5x-0711&rft.publisher=Commonwealth Scientific and Industrial Research Organisation&rft.description=Samples were collected from 4 harbour sites (Aus, NZ, USA & Canada) and sent to 12 labs for 18S and COI PCR. The dataset is the demultiplexed (by lab) data from this study.\nLineage: The source material was homogenised and give to 12 labs for DNA prep and PCR extraction/amplification using both fused and two-step primers. The resulting DNA was sequenced at Guelph and Curtin. The sequence files were then run together through a CSIRO amplicon pipeline so that differences between labs could be analysed and reproducibility asssesed.&rft.creator=Greenfield, Paul &rft.creator=Cawthron Institute &rft.creator=National Institute of Water and Atmospheric Research (NIWA) &rft.creator=University of Otago &rft.creator=University of Auckland &rft.creator=Curtin University &rft.creator=Macquarie University &rft.creator=Moss Landing Marine Laboratory &rft.creator=Department of Fisheries and Oceans &rft.creator=McGill University &rft.creator=University of Guelph &rft.creator=University of Oviedo &rft.creator=Klaipeda University &rft.date=2021&rft.edition=v4&rft_rights=CSIRO Data Licence https://research.csiro.au/dap/licences/csiro-data-licence/&rft_rights=Data is accessible online and may be reused in accordance with licence conditions&rft_rights=All Rights (including copyright) CSIRO, Cawthron Institute 2021.&rft_subject=metabarcoding&rft_subject=COI&rft_subject=18S&rft_subject=invasive species&rft_subject=reproducibility&rft_subject=amplicons&rft_subject=Conservation and Biodiversity&rft_subject=ENVIRONMENTAL SCIENCES&rft_subject=ENVIRONMENTAL SCIENCE AND MANAGEMENT&rft_subject=Environmental Management&rft_subject=Invasive Species Ecology&rft_subject=ECOLOGICAL APPLICATIONS&rft_subject=Environmental Monitoring&rft.type=dataset&rft.language=English Access the data
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Brief description

Samples were collected from 4 harbour sites (Aus, NZ, USA & Canada) and sent to 12 labs for 18S and COI PCR. The dataset is the demultiplexed (by lab) data from this study.
Lineage: The source material was homogenised and give to 12 labs for DNA prep and PCR extraction/amplification using both fused and two-step primers. The resulting DNA was sequenced at Guelph and Curtin. The sequence files were then run together through a CSIRO amplicon pipeline so that differences between labs could be analysed and reproducibility asssesed.

Available: 2021-04-22

Subjects
18S | COI | Conservation and Biodiversity | Ecological Applications | Environmental Science and Management | Environmental Sciences | Environmental Management | Environmental Monitoring | Invasive Species Ecology | amplicons | invasive species | metabarcoding | reproducibility |

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