Data

18S and COI sequence data from cross-lab reproducible metabarcoding study

Commonwealth Scientific and Industrial Research Organisation
Greenfield, Paul ; Cawthron Institute, New Zealand ; National Institute of Water and Atmospheric Research (NIWA), New Zealand ; University of Otago, New Zealand ; University of Auckland, New Zealand ; Curtin University, Australia ; Macquarie University, Australia ; Moss Landing Marine Laboratory, United States ; Department of Fisheries and Oceans, Canada ; McGill University, Canada ; University of Guelph, Canada ; University of Oviedo, Spain ; Klaipeda University, Lithuania
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25919/5j5x-0711&rft.title=18S and COI sequence data from cross-lab reproducible metabarcoding study&rft.identifier=10.25919/5j5x-0711&rft.publisher=Commonwealth Scientific and Industrial Research Organisation (CSIRO)&rft.description=Samples were collected from 4 harbour sites (Aus, NZ, USA & Canada) and sent to 12 labs for 18S and COI PCR. The dataset is the demultiplexed (by lab) data from this study.The source material was homogenised and give to 12 labs for DNA prep and PCR extraction/amplification using both fused and two-step primers. The resulting DNA was sequenced at Guelph and Curtin. The sequence files were then run together through a CSIRO amplicon pipeline so that differences between labs could be analysed and reproducibility asssesed.&rft.creator=Greenfield, Paul &rft.creator=Cawthron Institute, New Zealand &rft.creator=National Institute of Water and Atmospheric Research (NIWA), New Zealand &rft.creator=University of Otago, New Zealand &rft.creator=University of Auckland, New Zealand &rft.creator=Curtin University, Australia &rft.creator=Macquarie University, Australia &rft.creator=Moss Landing Marine Laboratory, United States &rft.creator=Department of Fisheries and Oceans, Canada &rft.creator=McGill University, Canada &rft.creator=University of Guelph, Canada &rft.creator=University of Oviedo, Spain &rft.creator=Klaipeda University, Lithuania &rft.date=2021&rft.edition=v4&rft_rights=All Rights (including copyright) CSIRO, Cawthron Institute 2021.&rft_rights=CSIRO Data Licence https://confluence.csiro.au/display/daphelp/CSIRO+Data+Licence&rft_subject=metabarcoding&rft_subject=COI&rft_subject=18S&rft_subject=invasive species&rft_subject=reproducibility&rft_subject=amplicons&rft_subject=Conservation and Biodiversity&rft_subject=ENVIRONMENTAL SCIENCES&rft_subject=ENVIRONMENTAL SCIENCE AND MANAGEMENT&rft_subject=Environmental Management&rft_subject=Invasive Species Ecology&rft_subject=ECOLOGICAL APPLICATIONS&rft_subject=Environmental Monitoring&rft.type=dataset&rft.language=English Access the data
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All Rights (including copyright) CSIRO, Cawthron Institute 2021.

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Brief description

Samples were collected from 4 harbour sites (Aus, NZ, USA & Canada) and sent to 12 labs for 18S and COI PCR. The dataset is the demultiplexed (by lab) data from this study.

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The source material was homogenised and give to 12 labs for DNA prep and PCR extraction/amplification using both fused and two-step primers. The resulting DNA was sequenced at Guelph and Curtin. The sequence files were then run together through a CSIRO amplicon pipeline so that differences between labs could be analysed and reproducibility asssesed.

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Subjects
18S | COI | Conservation and Biodiversity | Ecological Applications | Environmental Science and Management | Environmental Sciences | Environmental Management | Environmental Monitoring | Invasive Species Ecology | amplicons | invasive species | metabarcoding | reproducibility |

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